Preparation and characterization of solid lipid nanoparticles (SLN) made of cacao butter and curdlan
Preparation and characterization of solid lipid nanoparticles (SLN) made of cacao butter and curdlan
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DOI：
10.1016/j.ejps.2004.10.008
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发表时间：
2005-02-01
影响因子：
4.6
通讯作者：
Choi, HK
中科院分区：
文献类型：
--
作者：
Kim, BD;Na, K;Choi, HK
以可可脂为脂质核，凝乳蛋白为壳材料制备固体脂质纳米颗粒（SLN）。用吐温80作为助表面活性剂，防止凝乳的聚集和胶凝。甘露醇被用作冷冻保护剂，以防止在再分散过程中聚集。脂质浓度为1.0%时，SLN的粒径无明显变化，粒径范围为140 ~ 200 nm，呈单峰分布。当pH值在7和11之间交替测试SLN溶液的物理稳定性时，在脂质浓度为0.5%之前，粒径的变化保持在一个狭窄的范围内。在0.5%以上，由于涂层材料、胶凝剂和Tween 80用量不足，颗粒开始聚集。pH 7.4时的临界聚集浓度为6.95 × 10(-4) Mg/ml。用芘作为荧光探针。随着温度升高，芘逐渐从SLN中释放出来。维拉帕米与脂质比为1:10和1:5时，负载效率为0.75%，当维拉帕米与脂质比为1:1时，负载效率显著降低。维拉帕米进入SLN后，释放速度明显延迟。(C) 2004 Elsevier B.V.版权所有
Solid lipid nanoparticles (SLN) were prepared using cacao butter, as the lipid core, and curdlan, as the shell material. Tween 80 was used as a co-surfactant in order to prevent aggregation and gelling of the curdlan. Mannitol was used as a cryoprotectant in order to prevent aggregation during redispersion. No significant change in the size of the SLN was observed up to a lipid concentration of 1.0%, and the particle size ranged from 140 to 200 nm with a unimodal distribution. When an alternating pH between 7 and 11 was used to test the physical stability of an SLN solution, the change in the particle size remained within a narrow range up to a lipid concentration of 0.5%. Above 0.5%, the particles began to aggregate due to the insufficient amount of the coating material, curdlan and Tween 80. The critical aggregation concentration at pH 7.4 was found to be 6.95 x 10(-4) Mg/ml. Pyrene was used as a fluorescence probe. As the temperature increased, pyrene was gradually released from the SLN. The loading efficiency was >75% when the verapamil to lipid ratios were 1: 10 and 1:5 and decreased significantly as the ratio became 1: 1. The release rate was significantly delayed when verapamil was loaded into the SLN. (C) 2004 Elsevier B.V. All rights reserved.