Protein profiling of microdissected pancreas carcinoma and identification of HSP27 as a potential serum marker
Protein profiling of microdissected pancreas carcinoma and identification of HSP27 as a potential serum marker
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DOI：
10.1373/clinchem.2006.079194
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发表时间：
2007-04-01
影响因子：
9.3
通讯作者：
von Eggeling, Ferdinand
中科院分区：
文献类型：
--
作者：
Melle, Christian;Ernst, Guenther;von Eggeling, Ferdinand
背景：胰腺癌患者的预后很差，因为临床表现较晚，肿瘤具有侵袭性。我们使用蛋白质组学技术，以寻找胰腺癌的标志物。方法：我们进行了蛋白质分析的9胰腺癌和10个健康的胰腺组织样本，使用蛋白质芯片技术（表面增强激光解吸/电离）的microdisserted cryostat节。我们通过二维凝胶电泳、肽指纹图谱和免疫耗竭鉴定蛋白质，并使用免疫组织化学对所发现的蛋白质进行原位定位。我们使用ELISA对35名胰腺癌患者和37名健康个体的术前血清样本中的这些蛋白质进行定量。结果：从蛋白质芯片技术检测到的差异表达信号中，我们鉴定了2种蛋白质，DJ-1和热休克蛋白27（HSP 27）。然后，我们检测患者血清中的HSP 27通过使用ELISA，表明灵敏度为100%，特异性为84%的胰腺癌recognition of pancreatic cancer.Conclusions：DJ-1和HSP 27在纯定义的组织中的检测和血清中的HSP 27通过基于抗体的方法的检索确定了胰腺癌的潜在市场。(c)2007年美国临床化学协会。
Background: Patients with pancreatic adenocarcinomas have a poor prognosis because of late clinical manifestation and the tumor's aggressive nature. We used proteomic techniques to search for markers of pancreatic carcinoma.Methods: We performed protein profiling of microdissected cryostat sections of 9 pancreatic adenocarcinomas and 10 healthy pancreatic tissue samples using ProteinChip technology (surface-enhanced laser desorption/ionization). We identified proteins by use of 2-dimensional gel electrophoresis, peptide fingerprint mapping, and immunodepletion and used immunohistochemistry for in situ localization of the proteins found. We used ELISA to quantify these proteins in preoperative serum samples from 35 patients with pancreatic cancer and 37 healthy individuals.Results: From among the differentially expressed signals that were detected by ProteinChip technology, we identified 2 proteins, DJ-1 and heat shock protein 27 (HSP27). We then detected HSP27 in sera of patients by use of ELISA, indicating a sensitivity of 100% and a specificity of 84% for the recognition of pancreatic cancer.Conclusions: The detection of DJ-1 and HSP27 in pure defined tissue and the retrieval of HSP27 in serum by antibody-based methods identifies a potential market for pancreatic cancer. (c) 2007 American Association for Clinical Chemistry.