Functional role of arginine during the peri-implantation period of pregnancy. I. Consequences of loss of function of arginine transporter SLC7A1 mRNA in ovine conceptus trophectoderm
Functional role of arginine during the peri-implantation period of pregnancy. I. Consequences of loss of function of arginine transporter SLC7A1 mRNA in ovine conceptus trophectoderm
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DOI：
10.1096/fj.13-248757
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发表时间：
2014-07-01
期刊：
影响因子：
4.8
通讯作者：
Bazer, Fuller W.
中科院分区：
文献类型：
--
作者：
Wang, Xiaoqiu;Frank, James W.;Bazer, Fuller W.
精氨酸是哺乳动物产生一氧化氮（NO）和多胺的共同底物，在妊娠围着床期子宫腔内精氨酸含量增加。然而，精氨酸在子宫腔内对孕体（胚胎和胚外膜）发育的功能作用尚未在体内阐明。为了评估精氨酸在生殖组织中对孕体存活和发育的作用，我们进行了体内吗啉代反义寡核苷酸（MAO）介导的对绵羊孕体滋养外胚层（Tr）中精氨酸转运蛋白SLC 7A 1 mRNA的敲低。与MAO对照相比，SLC 7A 1 mRNA的翻译敲低导致胚胎发育迟缓和功能异常。在孕体中使用MAO-SLC 7A 1敲低降低精氨酸转运（73%，P
Arginine, the common substrate for production of nitric oxide (NO) and polyamines in mammals, increases in the uterine lumen during the peri-implantation period of pregnancy. However, functional roles of arginine within the uterine lumen for conceptus (embryo and extraembryonic membranes) development have not been elucidated in vivo. To assess roles of arginine in reproductive tissue for survival and development of the conceptus, we conducted an in vivo morpholino antisense oligonucleotide (MAO)-mediated knockdown of SLC7A1 mRNA, the arginine transporter in ovine conceptus trophectoderm (Tr). Translational knockdown of SLC7A1 mRNA resulted in retarded conceptus development and abnormal function compared to MAO control. Use of MAO-SLC7A1 knockdown in conceptuses decreased arginine transport (73%, P