Transcriptional and post-translational activation of AMPKα by oxidative, heat, and cold stresses in the red flour beetle, Tribolium castaneum
Transcriptional and post-translational activation of AMPKα by oxidative, heat, and cold stresses in the red flour beetle, Tribolium castaneum
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赤拟谷盗中氧化、热和冷应激对 AMPKα 的转录和翻译后激活
DOI：
10.1007/s12192-019-01030-x
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发表时间：
2019-11-01
影响因子：
3.8
通讯作者：
Wang, Jianjun
中科院分区：
文献类型：
--
作者：
Jiang, Heng;Zhang, Nan;Wang, Jianjun
腺苷酸活化蛋白激酶（AMPK）在能量代谢的调节中具有重要作用，AMPK的活性及其调控一直是相关研究的热点。然而，AMPK在昆虫中的功能表征仍然有限。本研究从赤拟谷盗Tribolium castaneum中克隆了编码AMPKα的全长cDNA（TcAMPKα）。TcAMPKα基因的开放阅读框（ORF）长1581 bp，编码526个氨基酸残基，与果蝇和哺乳动物同源基因具有相同的保守结构域。雌成虫暴露于氧化、热和冷应激导致TcAMPKα mRNA表达水平和激活环中Thr-173磷酸化水平增加。TcAMPKα的RNAi敲低导致T.板栗的氧化，热和冷应力。这些结果表明，应激信号调节TcAMPKα活性，TcAMPKα在实现保护机制和赋予对环境应激的抗性的过程中起着重要作用。
The AMP-activated protein kinase (AMPK) has important roles in the regulation of energy metabolism, and AMPK activity and its regulation have been the focus of relevant investigations. However, functional characterization of AMPK is still limited in insects. In this study, the full-length cDNA coding AMPKα (TcAMPKα) was isolated from the red flour beetle, Tribolium castaneum. The TcAMPKα gene contains an ORF of 1581 bp encoding a protein of 526 amino acid residues, which shared conserved domain structure with Drosophila melanogaster and mammalian orthologs. Exposure of female adults to oxidative, heat, and cold stresses caused an increase in TcAMPKα mRNA expression levels and phosphorylation of Thr-173 in the activation loop. The RNAi-mediated knockdown of TcAMPKα resulted in the increased sensitivity of T. castaneum to oxidative, heat, and cold stresses. These results suggest that stress signals regulate TcAMPKα activity, and TcAMPKα plays an important role in enabling protective mechanisms and processes that confer resistance to environmental stress.