Solute-solvent interactions on the surface of reversed phases
Solute-solvent interactions on the surface of reversed phases
复制标题
反相表面的溶质-溶剂相互作用
DOI：
10.1016/s0021-9673(00)80531-2
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发表时间：
1980
影响因子：
4.1
通讯作者：
C. Simpson
中科院分区：
文献类型：
--
作者：
R. Scott;C. Simpson
在流动相从纯甲醇到纯水发生突变的情况下，考察了商业上可用于高效液相色谱的几种“刷型”和“散型”反相柱填料的保留特性。结果表明，刷状反相在纯水中很难达到平衡，与水接触时，碳氢链表现为分散的相互作用。碳氢链的这种分散的分子内相互作用导致有效色层表面积的减少，从而导致异常低的保留特性。在与含有10%(w/v)或更多有机溶剂(如甲醇)的溶剂接触时，刷型材料表现出正常的保留特性。然而，体相或聚合物键合相与水迅速平衡，由于其更刚性的结构，似乎不表现出分散的分子内相互作用，因此表现出正常的保留特征。因此，块状或聚合型反相由于其更一致的层析性质，更适合用于检测溶质-溶剂/固定相相互作用。
The retention characteristics of a number of “brush”- and “bulk”-type reversed-phase column packing materials that are commercially available for high-performance liquid chromatography are examined in the situation where an abrupt change in mobile phase from pure methanol to pure water occurs. It is shown that the brush-type reversed phases are slow to come into equilibrium with pure water, and in contact with water it would appear that the hydrocarbon chains dispersively interact with themselves. This dispersive intramolecular interaction of the hydrocarbon chains results in a reduction in effective chromatographic surface area and consequently anomalously low retentive characteristics. In contact with solvent containing 10% (w/v) or more of organic solvent such as methanol, the brush-type materials exhibit normal retention characteristics. The bulk or polymeric bonded phases, however, equilibrate rapidly with water, and due to their more rigid structure do not appear to exhibit dispersive intramolecular interactions and thus exhibit normal retention characteristics. The bulk or polymeric type reversed phases, therefore, are to be preferred for examining solute-solvent/stationary phase interactions due to their more consistent chromatographic properties.