IMMUNOELECTRON MICROSCOPIC IDENTIFICATION AND LOCALIZATION OF STREPTOCOCCUS-SANGUIS WITH PEROXIDASE-LABELED ANTIBODY - LOCALIZATION OF STREPTOCOCCUS-SANGUIS IN INTACT DENTAL PLAQUE
IMMUNOELECTRON MICROSCOPIC IDENTIFICATION AND LOCALIZATION OF STREPTOCOCCUS-SANGUIS WITH PEROXIDASE-LABELED ANTIBODY - LOCALIZATION OF STREPTOCOCCUS-SANGUIS IN INTACT DENTAL PLAQUE
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DOI：
10.1128/iai.11.1.200-210.1975
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发表时间：
1975-01-01
影响因子：
3.1
通讯作者：
ROSAN, B
中科院分区：
文献类型：
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作者：
LAI, CH;LISTGARTEN, MA;ROSAN, B
利用辣根过氧化物酶标记抗体的间接技术，对血链球菌进行了超微结构定位。该技术允许试剂完全扩散到菌斑样本的所有部分。可以在菌斑的连续切片上进行控制程序，其细菌组成与实验样本的细菌组成相似。在切割超薄切片进行电子显微镜检查之前，可以在光学显微镜下检查30 μ m厚的切片，以定位用过氧化物酶特异性标记的区域。这项研究表明，特定的细菌可以定位在完整的牙菌斑。结果还表明，S.血细胞在牙菌斑中以垂直于牙齿表面的柱状小菌落生长。生长似乎是通过细胞分裂，而不是在表面沉积新细胞。尽管它们的结构保存相对较好，但与表面附近的细胞相比，斑块较深（较老）层中的细胞似乎已经失去了一些抗原活性。
Streptococcus sanguis has been localized ultrastructurally within intact dental plaque by means of an indirect technique which utilizes horseradish peroxidase-labeled antibody. The technique allows for complete diffusion of the reagents to all portions of the plaque specimens. Control procedures can be carried out on serial sections of plaque with a bacterial composition similar to that of the experimental specimen. The 30-mum-thick sections can be examined in the light microscope to localize areas specifically labeled with peroxidase prior to cutting ultra-thin sections for electron microscopy. This study demonstrated that specific bacteria can be localized within intact dental plaque. The results also indicated that S. sanguis grows in dental plaque as columnar shaped microcolonies perpendicular to the tooth surfaces. Growth appears to be by cell division rather than deposition of new cells at the surfaces. Despite their relatively good structural preservation, the cells in the deeper (older) layers of plaque appear to have lost some of their antigenic activity in comparison to the cells near the surface.