Light Potentials of Photosynthetic Energy Storage in the Field: What limits the ability to use or dissipate rapidly increased light energy?
Light Potentials of Photosynthetic Energy Storage in the Field: What limits the ability to use or dissipate rapidly increased light energy?
复制标题
现场光合能量储存的光势：是什么限制了使用或消散快速增加的光能的能力？
DOI：
10.1101/2021.08.26.457798
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Kramer, David M.
中科院分区：
文献类型：
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作者：
Kanazawa, Atsuko;Chattopadhyay, Abhijnan;Kuhlgert, Sebastian;Tuitupou, Hainite;Maiti, Tapabrata;Kramer, David M.
植物光合作用对环境条件快速波动的响应对于光能的有效转换至关重要。这些反应在实验室条件下并不常见，在野外环境中难以探测。我们展示了一个开放的科学方法来解决这个问题，结合了光合作用和环境条件的多方面测量，和一个无监督的统计聚类方法。在一组选定的关于薄荷的数据中，我们表明，“光潜力”的线性电子流和非光化学猝灭（NPQ）后，快速增加的光强烈抑制叶片先前暴露于低环境光合有效辐射（PAR）或低叶温，因素，可以独立和合作。进一步的分析使我们能够测试特定的机制。随着叶温或PAR的降低，在强光波动期间光合作用的限制从快速诱导的NPQ转移到光合作用控制的电子流在细胞色素b6f复合物。在低温下，强光诱导的流明酸化，但没有诱导NPQ，导致减少电子传递中间体的积累，可能诱导光损伤，揭示了一个潜在的目标，提高光合作用的效率和鲁棒性。我们讨论了开放科学的努力，以了解和提高作物生产力的方法的影响。
The responses of plant photosynthesis to rapid fluctuations in environmental conditions are critical for efficient conversion of light energy. These responses are not well-seen laboratory conditions and are difficult to probe in field environments. We demonstrate an open science approach to this problem that combines multifaceted measurements of photosynthesis and environmental conditions, and an unsupervised statistical clustering approach. In a selected set of data on mint (Menthasp.), we show that ‘light potentials’ for linear electron flow and non-photochemical quenching (NPQ) upon rapid light increases are strongly suppressed in leaves previously exposed to low ambient photosynthetically active radiation (PAR) or low leaf temperatures, factors that can act both independently and cooperatively. Further analyses allowed us to test specific mechanisms. With decreasing leaf temperature or PAR, limitations to photosynthesis during high light fluctuations shifted from rapidly induced NPQ to photosynthetic control of electron flow at the cytochromeb6fcomplex. At low temperatures, high light induced lumen acidification, but did not induce NPQ, leading to accumulation of reduced electron transfer intermediates, probably inducing photodamage, revealing a potential target for improving the efficiency and robustness of photosynthesis. We discuss the implications of the approach for open science efforts to understand and improve crop productivity.