Identifying Promoter Elements Necessary for Enamelin Tissue-Specific Expression
Identifying Promoter Elements Necessary for Enamelin Tissue-Specific Expression
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DOI：
10.1159/000151429
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发表时间：
2009-01-01
影响因子：
2.7
通讯作者：
Hu, Jan C. -C.
中科院分区：
文献类型：
--
作者：
Papagerakis, Petros;Hu, Yuanyuan;Hu, Jan C. -C.
釉质发育需要严格调控编码釉质基质蛋白基因的时空表达。在成釉的每个特定阶段，调控基因转录启动和终止的机制尚不清楚。在这项研究中，我们确定了正常的釉蛋白（Enam）的表达所需的顺式调控区域。Enam启动子5 '非编码区的序列分析鉴定了位于Enam翻译起始位点上游5.2 kb内的潜在重要的顺式调节元件。将含有Enam翻译起始位点上游5.2或3.9 kb的DNA构建体连接至LacZ报告基因并用于产生转基因小鼠。3.9kb Enam-LacZ转基因株在成釉细胞中不表达，但在成骨细胞中检测到异位LacZ染色。相反，5.2 kb Enam-LacZ构建体足以模拟内源性Enam成釉细胞特异性表达模式。我们的研究为Enam细胞和阶段特异性表达的分子控制提供了新的见解。版权所有（C）2008 S. Karger AG，巴塞尔
Enamel development requires the strictly regulated spatio-temporal expression of genes encoding enamel matrix proteins. The mechanisms orchestrating the initiation and termination of gene transcription at each specific stage of amelogenesis are unknown. In this study, we identify cis regulatory regions necessary for normal enamelin (Enam) expression. Sequence analysis of the Enam promoter 5'-non-coding region identified potentially important cis-regulatory elements located within 5.2 kb upstream of the Enam translation initiation site. DNA constructs containing 5.2 or 3.9 kb upstream of the Enam translation initiation site were linked to an LacZ reporter gene and used to generate transgenic mice. The 3.9-kb Enam-LacZ transgenic lines showed no expression in ameloblasts, but ectopic LacZ staining was detected in osteoblasts. In contrast, the 5.2-kb Enam-LacZ construct was sufficient to mimic the endogenous Enam ameloblast-specific expression pattern. Our study provides new insights into the molecular control of Enam cell- and stage-specific expression. Copyright (C) 2008 S. Karger AG, Basel