Cross-reactivity of human immunoglobulin G2 recognizing phosphorylcholine and evidence for protection against major bacterial pathogens of the human respiratory tract
Cross-reactivity of human immunoglobulin G2 recognizing phosphorylcholine and evidence for protection against major bacterial pathogens of the human respiratory tract
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DOI：
10.1086/424517
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发表时间：
2004-10-01
影响因子：
6.4
通讯作者：
Weiser, JN
中科院分区：
文献类型：
--
作者：
Goldenberg, HB;McCool, TL;Weiser, JN
磷酸胆碱（ChoP）是位于上呼吸道中的许多细菌和病原菌的细胞表面上的抗原组分。在本研究中，从合并的血清丙种球蛋白中亲和纯化人ChoP特异性抗体。这种天然获得的抗体主要是免疫球蛋白（IG）G2亚型，可识别肺炎链球菌脂磷壁酸和流感嗜血杆菌脂多糖上的ChoP，这两种物质是人类呼吸道感染的主要病原体。在体外杀伤试验中，抗ChoP IgG 2对一些临床分离的不可分型H.流感病毒和几种常见血清型的S.肺炎。此外，被动施用的人抗ChoP抗体保护小鼠免受透明分离的S.肺炎6A型。人类抗体对这种保守的细菌结构的有效性表明，如果可以操纵它以扩大其活性，它可以作为靶向多种病原体的单一疫苗抗原。
Phosphorylcholine (ChoP) is an antigenic component on the cell surface of many commensal and pathogenic bacteria that reside in the upper airway. In the present study, human ChoP-specific antibody was affinity-purified from pooled serum gamma globulin. This naturally acquired antibody, which is primarily of the immunoglobulin (Ig) G2 subtype, recognized ChoP on the lipoteichoic acid of Streptococcus pneumoniae and on the lipopolysaccharide of Haemophilus influenzae, 2 of the leading etiologic agents of infection involving the human respiratory tract. In in vitro killing assays, anti-ChoP IgG2 was effective against some clinical isolates of nontypeable H. influenzae and against isolates of several common serotypes of S. pneumoniae. Moreover, passively administered human anti-ChoP antibody protected mice against lethal challenge with a transparent isolate of S. pneumoniae type 6A. The effectiveness of human antibody to this conserved bacterial structure suggests that, if it can be manipulated to broaden its activity, it could function as a single vaccine antigen that targets multiple pathogens.