Effect of esterification condensation on the Folin-Ciocalteu method for the quantitative measurement of total phenols
Effect of esterification condensation on the Folin-Ciocalteu method for the quantitative measurement of total phenols
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DOI：
10.1016/j.foodchem.2014.08.038
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发表时间：
2015-03-01
期刊：
影响因子：
8.8
通讯作者：
Liang, Ji-Yuan
中科院分区：
文献类型：
--
作者：
Chen, Liang-Yu;Cheng, Chien-Wei;Liang, Ji-Yuan
Folin-Ciocalteu法是测定天然产物中总酚含量的常用方法。该方法受到添加碳酸钠的显着影响。可能对当前应用的Folin-Ciocalteu方法进行了修改，未对定量标准品和工艺顺序进行任何验证。在这项研究中，进行了一系列的实验，以探讨酚校准的影响的基础上，经典的Folin-Ciocalteu方法。酯化缩合，观察到在与前碱化没食子酸和儿茶素作为定量标准的测定。与没食子酸校准相比，样品中获得的酚含量不同，取决于何时发生碱化。本文首次对经典的Folin-Ciocalteu方法中分子交联引起的偏倚进行了定义。再次优化和验证了Folin-Ciocalteu方法的性能。皇冠版权所有（C）2014由爱思唯尔有限公司出版。保留所有权利。
The Folin-Ciocalteu method is widely applied for the determination of the total phenolic contents in natural products. This method is significantly affected by the addition of sodium carbonate. The currently applied Folin-Ciocalteu methods may have been modified without any validation in the quantitative standards and the order of processes. In this study, serial experiments were performed to investigate the effect of phenolic calibrations based on the classic Folin-Ciocalteu method. Esterification condensations were observed in the assays with prior basification for gallic acid and catechin used as quantitative standards. The phenolic contents obtained in the samples differed depending on when basification occurred compared with the gallic acid calibration. The bias of the classic Folin-Ciocalteu method derived from cross-linkage of molecules was first defined in this study. The performance of the Folin-Ciocalteu method is optimised and validated again. Crown Copyright (C) 2014 Published by Elsevier Ltd. All rights reserved.