Plasmodium vivax Duffy binding protein:: a modular evolutionary proposal
Plasmodium vivax Duffy binding protein:: a modular evolutionary proposal
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DOI：
10.1017/s0031182003004773
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发表时间：
2004-04-01
期刊：
影响因子：
2.4
通讯作者：
Patarroyo, MA
中科院分区：
文献类型：
--
作者：
Martinez, O;Suarez, CF;Patarroyo, MA
引起疟疾的寄生虫种群的特点是具有很大的遗传多样性。多态性产生机制的知识是开发有效的疟疾疫苗和了解寄生虫种群结构的核心问题。间日疟原虫的遗传多样性可以用两个主要因素来解释：自然选择和基因内重组。在本工作中，在间日疟原虫Duffy结合蛋白中发现了模块化组织。4个哥伦比亚分离株与Salvador-I株的dpb区III-VI序列完全一致，表明中南美洲分离株之间具有较高的同源性。地理上聚集的部门，对应于富含半胱氨酸的区域（II和VI），显示出高的序列多样性，可以反映可能的免疫应答逃避机制;在这些区域中检测到阳性和阴性选择。相反，其他dbp基因区域显示非地理聚集模式，较低的序列多样性和负选择压力占主导地位。在整个沿着分子上均能检测到疏水化。这些发现表明，多样性与多样性的力量，驱动DBP基因的进化，并确定其马赛克区域的组织。
The population of malaria-causing parasites is characterized by great genetic diversity. Knowledge of the polymorphism generation mechanism is a central issue for developing effective vaccines against malaria and understanding the parasite population structure. Plasmodium vivax genetic diversity has been explained in terms of two major factors : natural selection and intragenic recombination. A modular organization was found within P. vivax Duffy binding protein in the present work. Four Colombian isolates have identical sequences to Salvador-l strain amongst dpb regions III-VI analysed, suggesting a high identity between Central and South American isolates. Geographically clustered sectors, corresponding to cysteine-rich regions (II and VI), show a high sequence diversity that could reflect a possible immune response evasion mechanism; both positive and negative selection were detected in these regions. In contrast, other dbp gene regions display a non-geographical clustering pattern, lower sequence diversity and predominant negative selective pressure. Recombination was homogeneonsly detected all along the molecule. These findings suggest that diversification vs. homogenizing forces, drive dbp gene evolution and determine its mosaic region organization.