Correlation of bacterial lipid composition with antibiotic resistance
Correlation of bacterial lipid composition with antibiotic resistance
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DOI：
10.1128/jb.101.3.892-900.1970
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发表时间：
1970-03-01
影响因子：
3.2
通讯作者：
O'Leary, William M.
中科院分区：
文献类型：
--
作者：
Dunnick, June K.;O'Leary, William M.
细菌脂质组成与抗生素耐药性的相关性进行了研究，特别强调那些生物体的耐药性可能与膜或包膜结构或功能，如四环素和多粘菌素的耐药性。氯仿-甲醇可提取的脂质，磷脂酰乙醇胺馏分，这些脂质馏分和总脂肪酸的脂肪酸进行了研究，通过使用薄层色谱法，气相色谱法和红外光谱法。一致的定量差异之间的脂肪酸组成的敏感和耐药菌株。最值得注意的是，在革兰氏阴性菌中，耐药菌株与敏感菌株相比，环丙烷酸减少。发现这些变化是菌株固有的，而不是由于生长阶段或培养时间。未观察到显著的质量差异。相反，在青霉素敏感和耐药的革兰氏阳性球菌菌株中没有观察到脂肪酸含量的这种变化。由于脂肪酸组成的显着改变，指出在革兰氏阴性菌株耐抗生素，我们认为，电阻是相关的膜或包膜脂质组成。
The correlation of bacterial lipid composition with antibiotic resistance was investigated with particular emphasis on those organisms in which resistance may be related to membrane or envelope structure or function, as in resistance to tetracyclines and polymyxin. Chloroform-methanol-extractable lipids, phosphatidyl ethanolamine fractions, and both fatty acids of these lipid fractions and total fatty acids were studied by using thin-layer chromatography, gas chromatography, and infrared spectroscopy. Consistent quantitative differences were found between the fatty acid compositions of sensitive and resistant strains. Most notable was the fact that, in gram-negative organisms, resistant strains showed decreases in cyclopropane acids as compared with sensitive strains. These changes were found to be inherent in the strains and not due to growth stage or culture age. No significant qualitative differences were noted. In contrast, no such variation in fatty acid content was observed in penicillin-sensitive and resistant strains of gram-positive cocci. As significant alterations of fatty acid composition were noted in gram-negative strains resistant to antibiotics, we suggest that resistance is correlated to membrane or envelope lipid composition.