Analysis of the 5'UTR of HCV genotype 3 grown in vitro in human B cells, T cells, and macrophages
Analysis of the 5'UTR of HCV genotype 3 grown in vitro in human B cells, T cells, and macrophages
复制标题
在人 B 细胞、T 细胞和巨噬细胞中体外生长的 HCV 基因型 3 的 5UTR 分析
作者：
D. Revie;Michael O. Alberti;Michael O. Alberti;John G Prichard;A. Kelley;S. Z. Salahuddin
背景我们曾报道从感染者中分离出基因型为1的人丙型肝炎病毒（HCV-1），并对其进行了分子鉴定。我们现在报告的HCV基因型3（HCV-3）的诊断由临床laborators.ResultsHCV感染的患者获得的分析在体外培养使用我们的系统。从患者的血液和在各种细胞类型中培养长达三个月的HCV中分离HCV RNA。这些分离株的5 'UTR用于比较。结果显示，与血清RNA相比，有许多序列变化。由感染的巨噬细胞、B细胞和T细胞有效产生的HCV RNA具有与HCV-1相似的序列，这表明在巨噬细胞水平上进行变体的选择。与HCV-1相似序列的病毒在巨噬细胞中的复制能力强于与HCV-3相似序列的病毒。结论HCV-3虽然在B细胞、T细胞和巨噬细胞中复制，但可能需要不同的原代细胞才能复制。因此，我们认为，HCV-3不能在巨噬细胞中有效复制，感染HCV-3的患者血液中可能含有HCV-1。
BackgroundPreviously, we have reported the isolation and molecular characterization of human Hepatitis C virus genotype 1 (HCV-1) from infected patients. We are now reporting an analysis of HCV obtained from patients infected with HCV genotype 3 (HCV-3) as diagnosed by clinical laboratories.ResultsHCV was cultured in vitro using our system. HCV RNA was isolated from patients' blood and from HCV cultured in various cell types for up to three months. The 5'UTR of these isolates were used for comparisons. Results revealed a number of sequence changes as compared to the serum RNA. The HCV RNA produced efficiently by infected macrophages, B-cells, and T-cells had sequences similar to HCV-1, which suggests that selection of the variants was performed at the level of macrophages. Virus with sequences similar to HCV-1 replicated better in macrophages than HCV having a 5'UTR similar to HCV-3.ConclusionsAlthough HCV-3 replicates in cell types such as B-cells, T-cells, and macrophages, it may require a different primary cell type for the same purpose. Therefore, in our opinion, HCV-3 does not replicate efficiently in macrophages, and patients infected with HCV-3 may contain a population of HCV-1 in their blood.
DOI：
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发表时间：
2002
期刊：
NIH consensus and state-of-the-science statements
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