Spatiotemporal localization of D-amino acid oxidase and D-aspartate oxidases during development in Caenorhabditis elegans
Spatiotemporal localization of D-amino acid oxidase and D-aspartate oxidases during development in Caenorhabditis elegans
复制标题
秀丽隐杆线虫发育过程中 D-氨基酸氧化酶和 D-天冬氨酸氧化酶的时空定位
DOI：
10.1128/mcb.06513-11
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发表时间：
2012
期刊：
影响因子：
5.3
通讯作者：
Homma H
中科院分区：
文献类型：
--
作者：
Saitoh Y;Katane M;Kawata T;Maeda K;Sekine M;Furuchi T;Kobuna H;Sakamoto T;Inoue T;Arai H;Nakagawa Y;Homma H
近年来的研究表明，d-氨基酸在生物体中广泛存在，并可能在机体的生理功能中发挥重要作用，d-氨基酸氧化酶（DAO）和d-天冬氨酸氧化酶（DDO）是立体专一性降解d-氨基酸的酶。它们已经在各种生物体中被鉴定，包括哺乳动物和线虫线虫，尽管这些酶的重要性和d-氨基酸的相关功能仍有待阐明。在这项研究中，我们研究了时空定位的C。elegans的DAO和DDO（DDO-1、DDO-2和DDO-3），并测量野生型C中几种α-和β-氨基酸的水平。elegans和四个突变体，其中每个DAO和DDO基因部分缺失，从而失活。此外，这些突变株的几个表型进行了表征。结果表明，C. DAO和DDO参与了产蛋事件和C.优美的特别是，DDO似乎在生殖细胞的发育和成熟中发挥重要作用。这项工作提供了新的和有用的见解，这些酶和d-氨基酸在多细胞生物的生理功能。
Recent investigations have shown that a variety ofd-amino acids are present in living organisms and that they possibly play important roles in physiological functions in the body.d-Amino acid oxidase (DAO) andd-aspartate oxidase (DDO) are degradative enzymes stereospecific ford-amino acids. They have been identified in various organisms, including mammals and the nematodeCaenorhabditis elegans, although the significance of these enzymes and the relevant functions ofd-amino acids remain to be elucidated. In this study, we investigated the spatiotemporal localization ofC. elegansDAO and DDOs (DDO-1, DDO-2, and DDO-3) and measured the levels of severald- andl-amino acids in wild-typeC. elegansand four mutants in which each gene for DAO and the DDOs was partially deleted and thereby inactivated. Furthermore, several phenotypes of these mutant strains were characterized. The results reported in this study indicate thatC. elegansDAO and DDOs are involved in egg-laying events and the early development ofC. elegans. In particular, DDOs appear to play important roles in the development and maturation of germ cells. This work provides novel and useful insights into the physiological functions of these enzymes andd-amino acids in multicellular organisms.