CHROMOSOMAL POSITION OR VIRUS MUTATION PERMITS RETROVIRUS EXPRESSION IN EMBRYONAL CARCINOMA-CELLS
CHROMOSOMAL POSITION OR VIRUS MUTATION PERMITS RETROVIRUS EXPRESSION IN EMBRYONAL CARCINOMA-CELLS
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DOI：
10.1016/0092-8674(86)90596-9
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发表时间：
1986-11-07
期刊：
影响因子：
64.5
通讯作者：
JAENISCH, R
中科院分区：
文献类型：
--
作者：
BARKLIS, E;MULLIGAN, RC;JAENISCH, R
逆转录病毒的表达仅限于胚胎癌细胞（EC）。为了研究病毒如何克服这种阻碍，我们选择并分析了在 F9 细胞中表达的稀有原病毒。我们的结果表明，原病毒表达通过两种不同的机制发生：一种原病毒在逆转录病毒tRNA引物结合位点获得单碱基对突变，从而允许原病毒表达；另一种是原病毒在逆转录病毒tRNA引物结合位点获得单碱基对突变，从而允许原病毒表达。三种原病毒的表达由5''-侧翼DNA序列介导。令人惊讶的是，17 个选定的细胞系中的 5 个原病毒整合到相同的两个不同的染色体区域中，这表明细胞基因组中允许病毒表达的染色体位置的数量非常有限。我们的结果表明，在 EC 细胞中活跃转录的基因组序列可以通过选择逆转录病毒表达来分离。
Retrovirus expression is restricted in embryonal carcinoma (EC) cells. To study how a virus can overcome this block, we selected and analyzed rare proviruses that are expressed in F9 cells. Our results indicate that provirus expression occurs by two different mechanisms: one provirus acquired a single base pair mutation in the retrovirus tRNA primer binding site, permitting provirus expression; expression of three proviruses was mediated by 5''-flanking DNA sequences. Surprisingly, five proviruses in 17 selected cell lines integrated into the same two distinct chromosomal regions, suggesting that the number of chromosomal positions in the cellular genome that allows virus expression is very limited. Our results suggest that genomic sequences that are actively transcribed in EC cells can be isolated by selection for retrovirus expression.