A polyethylenimine-mimetic biodegradable polycation gene vector and the effect of amine composition in transfection efficiency.
A polyethylenimine-mimetic biodegradable polycation gene vector and the effect of amine composition in transfection efficiency.
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DOI：
10.1016/j.biomaterials.2013.02.068
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发表时间：
2013-06
期刊：
影响因子：
14
通讯作者：
J. Shen;D. Zhao;W. Li;Q. Hu;Q. Wang;F. Xu;G. Tang
中科院分区：
文献类型：
--
作者：
J. Shen;D. Zhao;W. Li;Q. Hu;Q. Wang;F. Xu;G. Tang
聚合物载体的低毒性和高效的基因传递仍然是非病毒基因治疗临床应用的主要障碍。在这里，我们提出了一种基于聚d， l-琥珀酰亚胺（PSI）的可生物降解阳离子聚合物，它模仿了黄金标准，支化聚乙烯亚胺（PEI, ~ 25 kDa）。为了研究1°、2°、3°胺基比对聚合物的影响，我们首先对一系列接枝N，N-二甲基二丙基三胺（NN′）和双（3-氨基丙基）胺（NN）不同胺侧链的PSI-NN′- nny载体进行了表征和生物物理测量对比。体外和体内生物实验表明，PSI-NN ' 0.85- nn1具有比PEI更好的转染能力和生物相容性。本研究结果表明，这种模拟pei的可生物降解PSI-NN ' 0.85- nn1在临床基因传递方面具有良好的应用前景。
The low toxicity and efficient gene delivery of polymeric vectors remain the major barrier to the clinical application of non-viral gene therapy. Here, we present a poly-d, l-succinimide (PSI)-based biodegradable cationic polymer which mimicked the golden standard, branched polyethylenimine (PEI, ∼25 kDa). To investigate the influence of 1°, 2°, 3° amine group ratio in the polymer, a series of PSI-based vectors (PSI-NN′x-NNy) grafted with different amine side chains of N,N-dimethyldipropylenetriamine (NN’) and bis(3-aminopropyl)amine (NN) were first characterized and contrasted by biophysical measurements. The in vitro and in vivo biological assay demonstrated that PSI-NN′0.85-NN1exhibited better transfection ability and biocompatibility than PEI. The present results suggest that such PEI-mimic biodegradable PSI-NN′0.85-NN1possesses a good potential application for clinical gene delivery.