ImmunoFET feasibility in physiological salt environments
ImmunoFET feasibility in physiological salt environments
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DOI：
10.1098/rsta.2011.0503
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发表时间：
2012-05-28
期刊：
影响因子：
5
通讯作者：
Lee, Stephen C.
中科院分区：
文献类型：
--
作者：
Casal, Patricia;Wen, Xuejin;Lee, Stephen C.
场效应晶体管（FET）是固态电子器件，其特征在于电流源、电流漏和电荷载流子迁移通过的半导体沟道。FET可以是便宜的，检测分析物而无需标记，对由分析物结合介导的表面电位变化表现出指数响应，需要有限的样品制备并且真实的实时操作。用于蛋白质感测的免疫FET在其通道（抗体）上部署生物亲和元件，分析物结合到其调节免疫FET电特性。历史上，由于生理环境中的离子屏蔽，免疫FET被评估为不可行。我们证明了可靠的免疫场效应晶体管传感趋化因子相对离子不渗透的III族氮化物免疫场效应晶体管（异质结场效应晶体管）在生理缓冲液。数据显示，检测的特异性遵循用作受体的抗体的特异性，使我们能够区分个体高度相关的蛋白质种类（人和鼠CXCL9）以及分析物的混合样品（天然和生物素化CXCL9）。这些能力表明，免疫HFET可以是可行的，相反，经典的FET传感评估。FET蛋白质传感器可能导致比临床，生物技术和环境应用中的免疫测定更快，更便宜的即时诊断。
Field-effect transistors (FETs) are solid-state electrical devices featuring current sources, current drains and semiconductor channels through which charge carriers migrate. FETs can be inexpensive, detect analyte without label, exhibit exponential responses to surface potential changes mediated by analyte binding, require limited sample preparation and operate in real time. ImmunoFETs for protein sensing deploy bioaffinity elements on their channels (antibodies), analyte binding to which modulates immunoFET electrical properties. Historically, immunoFETs were assessed infeasible owing to ion shielding in physiological environments. We demonstrate reliable immunoFET sensing of chemokines by relatively ion-impermeable III-nitride immunoHFETs (heterojunction FETs) in physiological buffers. Data show that the specificity of detection follows the specificity of the antibodies used as receptors, allowing us to discriminate between individual highly related protein species (human and murine CXCL9) as well as mixed samples of analytes (native and biotinylated CXCL9). These capabilities demonstrate that immunoHFETs can be feasible, contrary to classical FET-sensing assessment. FET protein sensors may lead to point-of-care diagnostics that are faster and cheaper than immunoassay in clinical, biotechnological and environmental applications.