Expression of the Streptomyces aureofaciens glyceraldehyde-3-phosphate dehydrogenase gene (gap) is developmentally regulated and induced by glucose
Expression of the Streptomyces aureofaciens glyceraldehyde-3-phosphate dehydrogenase gene (gap) is developmentally regulated and induced by glucose
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DOI：
10.1099/00221287-143-11-3555
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发表时间：
1997-11-01
期刊：
影响因子：
--
通讯作者：
Homerova, D
中科院分区：
文献类型：
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作者：
Kormanec, J;Lempelova, A;Homerova, D
在先前的实验中，鉴定了编码甘油醛-3-磷酸脱氢酶（GAPDH）的金色链霉菌gap基因。为了研究该基因的表达，使用从S.在各种条件下培养的金霉素。这些研究表明，单顺反子组织和发育调控的基因。在差距编码区上游鉴定出单个启动子gap-P。在不存在葡萄糖的固体培养基上生长的培养物中，其转录在气生菌丝体形成时被诱导。此外，葡萄糖也诱导了底物菌丝体中的gap转录。将带有启动子的DNA片段插入两个启动子探针载体中，以得到与直接RNA分析结果一致的表达模式。
In previous experiments, the Streptomyces aureofaciens gap gene encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified. To investigate expression of the gene, S1 nuclease mapping and Northern blot hybridization were performed using RNA prepared from S. aureofaciens cultivated under various conditions. These studies suggested monocistronic organization and developmental regulation of the gene. A single promoter, gap-P, was identified upstream of the gap coding region. In cultures grown on solid medium in the absence of glucose, its transcription was induced at the time of aerial mycelium formation. In addition, gap transcription was also induced in substrate mycelium by glucose. A promoter-bearing DNA fragment was inserted into two promoter-probe vectors, to give expression patterns consistent with the results of direct RNA analysis.