A large family of Dscam genes with tandemly arrayed 5' cassettes in Chelicerata.
A large family of Dscam genes with tandemly arrayed 5' cassettes in Chelicerata.
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Chelicerata 中具有串联排列的 5' 盒的 Dscam 基因大家族
DOI：
10.1038/ncomms11252
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发表时间：
2016-04-15
影响因子：
16.6
通讯作者：
Jin Y
中科院分区：
文献类型：
--
作者：
Yue Y;Meng Y;Ma H;Hou S;Cao G;Hong W;Shi Y;Guo P;Liu B;Shi F;Yang Y;Jin Y
果蝇Dscam1(唐氏综合症细胞黏附分子)和脊椎动物簇生原钙粘附素(Pcdhs)是单一基因组座位异构体多样性的两个经典例子。Dscam1通过相互排斥的剪接编码38,016种不同的亚型，而脊椎动物簇生的Pcdhs利用不同的启动子来产生亚型多样性。在这里，我们揭示了一个缩短的DSCAM基因家族，其5‘端排列的盒式磁带排列在螯合目中。这些盒重复序列通常包括两个或四个外显子，对应于果蝇Dscam1的可变免疫球蛋白7(Ig7)或Ig7-8结构域。此外，通过交替启动子和选择性剪接的组合，产生了非凡的异构体多样性。这些sDscam与果蝇Dscam1有很高的序列相似性，并与脊椎动物聚集性Pcdhs的5‘端可变区在组织上有显著的相似性。因此，我们的发现对于理解果蝇Dscam1和脊椎动物Pcdhs之间的功能相似性具有重要意义，并可能为同工型多样性的调控提供进一步的机械性见解。果蝇Dscam1和脊椎动物簇生原钙粘附素(Pcdh)以其非凡的异构体多样性而闻名。在这里，作者在chelicerata中发现了一个缩短的Dscam基因家族，它与果蝇Dscam1同源，并采用了类似于脊椎动物Pcdhs的剪接模式。
Drosophila Dscam1 (Down Syndrome Cell Adhesion Molecules) and vertebrate clustered protocadherins (Pcdhs) are two classic examples of the extraordinary isoform diversity from a single genomic locus. Dscam1 encodes 38,016 distinct isoforms via mutually exclusive splicing in D. melanogaster, while the vertebrate clustered Pcdhs utilize alternative promoters to generate isoform diversity. Here we reveal a shortened Dscam gene family with tandemly arrayed 5′ cassettes in Chelicerata. These cassette repeats generally comprise two or four exons, corresponding to variable Immunoglobulin 7 (Ig7) or Ig7–8 domains of Drosophila Dscam1. Furthermore, extraordinary isoform diversity has been generated through a combination of alternating promoter and alternative splicing. These sDscams have a high sequence similarity with Drosophila Dscam1, and share striking organizational resemblance to the 5′ variable regions of vertebrate clustered Pcdhs. Hence, our findings have important implications for understanding the functional similarities between Drosophila Dscam1 and vertebrate Pcdhs, and may provide further mechanistic insights into the regulation of isoform diversity. Drosophila Dscam1 and vertebrate clustered protocadherins (Pcdh) are known for their extraordinary isoform diversity. Here authors identify a shortened Dscam gene family in Chelicerata, which displays homology to Drosophila Dscam1, and employs splicing patterns similar to that of vertebrate Pcdhs.