A novel three-dimensional variant of the watershed transform for segmentation of electron density maps
A novel three-dimensional variant of the watershed transform for segmentation of electron density maps
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DOI：
10.1016/s1047-8477(02)00009-6
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发表时间：
2002-04-01
影响因子：
3
通讯作者：
Volkmann, N
中科院分区：
文献类型：
--
作者：
Volkmann, N
由于缺乏可识别的特征，中等分辨率的电子密度图通常难以解释。这对于电子层析图来说尤其如此，它除了受到低信噪比的分辨率限制外，还受到分辨率限制。将这种地图可靠地分割成更小的、可管理的单元，可以极大地促进解释。在这里，我们提出了一种针对电子显微镜导出的三维电子密度图的分割方法。该方法由一种新的基于浸泡的分水岭算法的三维变体组成。我们在计算数据上测试了该算法，并将其应用于各种电子密度图，从单个大分子的重建到亚细胞结构的层析成像。结果表明，该算法可靠、高效、准确，适用于各种生物问题。(C) 2002 Elsevier Science （USA）。版权所有。
Electron density maps at moderate resolution are often difficult to interpret due to the lack of recognizable features. This is especially true for electron tomograms that suffer in addition to the resolution limitation from low signal-to-noise ratios. Reliable segmentation of such maps into smaller, manageable units can greatly facilitate interpretation. Here, we present a segmentation approach targeting three-dimensional electron density maps derived by electron microscopy. The approach consists of a novel three-dimensional variant of the immersion-based watershed algorithm. We tested the algorithm on calculated data and applied it to a wide variety of electron density maps ranging from reconstructions of single macromolecules to tomograms of subcellular structures. The results indicate that the algorithm is reliable, efficient, accurate, and applicable to a wide variety of biological problems. (C) 2002 Elsevier Science (USA). All rights reserved.