The anterior esophageal region of Schistosoma japonicum is a secretory organ.
The anterior esophageal region of Schistosoma japonicum is a secretory organ.
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日本血吸虫的食管前区是分泌器官
DOI：
10.1186/s13071-014-0565-8
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发表时间：
2014-12-10
影响因子：
3.2
通讯作者：
Wilson RA
中科院分区：
文献类型：
--
作者：
Li XH;Stark M;Vance GM;Cao JP;Wilson RA
背景：尽管几十年前就将其后部指定为腺，但其食管在很大程度上被忽视了。然而，我们最近表明它在血液处理中发挥着关键作用。它被清楚地划分为前室和后室，两者都被大量的细胞体包围。喂食电影显示，红细胞在进入后室之前在前室中积累，表明在那里执行了一个独特的过程。因此，我们调查的超微结构方面和可能的功能的前region.Methods：成人日本血吸虫的头部分离和准备的透射和扫描电子显微镜，以确定详细的超微结构的前食道。冷冻切片的头部也准备了免疫细胞化学和共聚焦显微镜，以确定固有的宿主抗体结合在前食管lining.Results的模式：食管的前合胞体衬里是高度延伸的长，薄波纹的细胞质向管腔突出。在雄虫中，波纹的尖端被大量的细胞质线进一步扩张，在中央管腔中产生意大利面条样的外观。扁平的、有凹痕的细胞质板散布在缠结的丝状物中。丰富的，形态不同的光泡不同的大小和内容物中制造的细胞体，从那里他们交通通过细胞质连接的波纹和尖端。雄性的囊泡聚集在扩展的尖端，偶尔有线粒体，而雌性有更多的线粒体，但囊泡较少。轻囊泡的膜质内容物主要从顶端分泌，但也从皱褶的侧面分泌。它们覆盖表面，然后在脱落到管腔中时形成有组织的自粘附膜。宿主抗体强烈结合在一个特征性的模式，食管前壁的分泌物是高度免疫原性的。结论：我们认为，食管前区是一个独立的分泌器官。轻囊泡的内容物通过食管的尖端释放到食管腔中，与进入的血液相互作用。我们的当务之急是确定它们的组成和在血液处理中的作用。
Background:The esophagus of blood-feeding schistosomes has been largely neglected although its posterior portion was designated as a gland decades ago. However, we recently showed it plays a pivotal role in blood processing. It is clearly demarcated into anterior and posterior compartments, both surrounded by a mass of cell bodies. Feeding movies revealed that erythrocytes accumulate in the anterior compartment before entering the posterior, indicating that a distinct process is executed there. We therefore investigated ultrastructural aspects and possible functions of the anterior region.Methods:The heads of adult Schistosoma japonicum were detached and prepared for both transmission and scanning electron microscopy to define the detailed ultrastructure of the anterior esophagus. Cryosections of heads were also prepared for immunocytochemistry and confocal microscopy to define the pattern of intrinsic host antibody binding in the anterior esophageal lining.Results:The anterior syncytial lining of the esophagus is highly extended by long, thin corrugations of cytoplasm projecting towards the lumen. Strikingly in the male worm, the tips of the corrugations are further expanded by numerous threads of cytoplasm, producing a spaghetti-like appearance in the central lumen. Flattened, pitted cytoplasmic plates are interspersed in the tangled mass of threads. Abundant, morphologically distinct light vesicles of varied size and contents are manufactured in the cell bodies, from where they traffic through cytoplasmic connections to the corrugations and out to the tips. Clusters of vesicles accumulate in expanded tips in males, together with occasional mitochondria whilst females have more mitochondria but fewer vesicles. The membranous contents of light vesicles are secreted mainly from the tips, but also from the sides of the corrugations. They coat the surfaces and then form organised self-adherent membrane figures when shed into the lumen. Host antibody binds strongly in a characteristic pattern to the anterior esophageal lining indicating that the secretions are highly immunogenic.Conclusions:We suggest that the anterior esophageal region is an independent secretory organ. The contents of light vesicles are released into the esophageal lumen via the tips of corrugation to interact with incoming blood. Our immediate task is to establish their composition and role in blood processing.
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影响因子：
3.8
作者：
Li XH;de Castro-Borges W;Parker-Manuel S;Vance GM;Demarco R;Neves LX;Evans GJ;Wilson RA
通讯作者：
Wilson RA
影响因子：
2
作者：
NORDESTGAARD, BG;ROSTGAARD, J
通讯作者：
ROSTGAARD, J
影响因子：
1.3
作者：
VOGE, M;PRICE, Z;BRUCKNER, DA
通讯作者：
BRUCKNER, DA
影响因子：
1.3
作者：
MORRIS, GP;THREADGOLD, LT
通讯作者：
THREADGOLD, LT
影响因子：
9.6
作者：
He, YX;Salafsky, B;Ramaswamy, K
通讯作者：
Ramaswamy, K