Classification using hierarchical clustering of tumor-infiltrating immune cells identifies poor prognostic ovarian cancers with high levels of COX expression
Classification using hierarchical clustering of tumor-infiltrating immune cells identifies poor prognostic ovarian cancers with high levels of COX expression
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DOI：
10.1038/modpathol.2008.187
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发表时间：
2009-03-01
期刊：
影响因子：
7.5
通讯作者：
Konishi, Ikuo
中科院分区：
文献类型：
--
作者：
Liu, Min;Matsumura, Noriomi;Konishi, Ikuo
局部免疫状态受肿瘤微环境的影响。本研究旨在通过检测肿瘤浸润性免疫细胞以及肿瘤细胞中环氧合酶（COX）的表达来表征局部免疫/微环境状态，并分析其与卵巢癌预后的关系。免疫组化染色70例卵巢癌标本，计数CD8+、CD57+、CD1a+细胞浸润上皮内或间质间隙的数量（6个参数）。采用分层聚类方法对6个参数进行一次分析。免疫组化分析肿瘤细胞中COX-1和COX-2的表达。COX-1和COX-2的表达与上皮内CD8+细胞呈负相关(P
Local immune status is influenced by the tumor microenvironment. This study aims to characterize the local immune/microenvironment status by examining tumor-infiltrating immune cells, as well as cyclooxygenase (COX) expression in tumor cells, and to analyze the relationship with the prognosis of ovarian cancers. Using immunohistochemical staining of 70 ovarian cancer specimens, the numbers of CD8+, CD57+, and CD1a+ cells infiltrating intraepithelial or stromal spaces were counted (six parameters). Hierarchical clustering was used to analyze the six parameters at one time. Expression of COX-1 and COX-2 in tumor cells was also analyzed by immunohistochemistry. Expression of both COX-1 and COX-2 was negatively correlated with intraepithelial CD8+ cells (P