Positive control of pre-mRNA splicing in vitro.
Positive control of pre-mRNA splicing in vitro.
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DOI：
10.1126/science.1566072
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发表时间：
1992-04
期刊：
影响因子：
56.9
通讯作者：
M. Tian;Tom Maniatis
中科院分区：
文献类型：
--
作者：
M. Tian;Tom Maniatis
果蝇性别特异性选择性剪接的前体信使RNA（pre-mRNA）的阳性调控涉及Transformer（tra）和Transformer-2（tra-2）基因产物对雌性特异性3'剪接位点的激活。在体外系统中研究了这一过程的机制，其中雌性特异性3'剪接位点可以被重组Tra或Tra-2（或两者）激活。体内调控所必需的外显子序列被证明是体外激活所必需和充分的。发现除了Tra和Tra-2之外的核蛋白特异性结合该外显子序列。因此，Tra和Tra-2可能通过促进外显子序列上的多蛋白复合物的组装而起作用。该复合物可促进剪接机器对相邻3'剪接位点的识别。
Positive control of the sex-specific alternative splicing of doublesex (dsx) precursor messenger RNA (pre-mRNA) in Drosophila melanogaster involves the activation of a female-specific 3' splice site by the products of the transformer (tra) and transformer-2 (tra-2) genes. The mechanisms of this process were investigated in an in vitro system in which the female-specific 3' splice site could be activated by recombinant Tra or Tra-2 (or both). An exon sequence essential for regulation in vivo was shown to be both necessary and sufficient for activation in vitro. Nuclear proteins in addition to Tra and Tra-2 were found to bind specifically to this exon sequence. Therefore, Tra and Tra-2 may act by promoting the assembly of a multiprotein complex on the exon sequence. This complex may facilitate recognition of the adjacent 3' splice site by the splicing machinery.