Printing Therapeutic Proteins in 3D using Nanoengineered Bioink to Control and Direct Cell Migration
Printing Therapeutic Proteins in 3D using Nanoengineered Bioink to Control and Direct Cell Migration
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DOI：
10.1002/adhm.201801553
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发表时间：
2019-06-01
影响因子：
10
通讯作者：
Gaharwar, Akhilesh K.
中科院分区：
文献类型：
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作者：
Peak, Charles W.;Singh, Kanwar Abhay;Gaharwar, Akhilesh K.
一种装载治疗性蛋白质的纳米工程生物链接被设计用于在3D打印结构中指导细胞功能。这种生物链接是由一种可水解降解的聚合物和二维合成纳米颗粒制成的。聚乙二醇-二硫苏糖醇（PEGDTT）的合成采用Michael-like阶跃生长聚合，得到丙烯酸酯端部可降解高分子。在peggdtt中加入二维纳米硅酸盐，形成了具有高印刷性和结构保真度的剪切变薄生物墨水。通过改变PEG:PEGDTT的比例和纳米硅酸盐的浓度，可以调节3D打印结构体的力学性能、膨胀动力学和降解速率。由于纳米硅酸盐的高表面积和带电特性，蛋白质治疗剂可以在3D打印结构中隔离更长时间。从3D打印结构中持续释放促血管生成疗法，促进人脐静脉内皮细胞的快速迁移。这种设计生物活性墨水来控制和指导细胞行为的方法可用于设计用于再生医学的3D复杂组织结构。
A nanoengineered bioink loaded with therapeutic proteins is designed to direct cell function in a 3D printed construct. The bioink is developed from a hydrolytically degradable polymer and 2D synthetic nanoparticle. The synthesis of poly(ethylene glycol)-dithiothreitol (PEGDTT) via a Michael-like step growth polymerization results in acrylate terminated degradable macromer. The addition of 2D nanosilicates to PEGDTT results in formation of shear-thinning bioinks with high printability and structural fidelity. The mechanical properties, swelling kinetics, and degradation rate of 3D printed constructs can be modulated by changing the ratio of PEG:PEGDTT and nanosilicates concentration. Due to high surface area and charged characteristic of nanosilicates, protein therapeutics can be sequestered in 3D printing structure for prolong duration. Sustained release of pro-angiogenic therapeutics from 3D printed structure, promoted rapid migration of human endothelial umbilical vein cell. This approach to design biologically active inks to control and direct cell behavior can be used to engineer 3D complex tissue structure for regenerative medicine.