MISSPLICING YIELDS CIRCULAR RNA MOLECULES
MISSPLICING YIELDS CIRCULAR RNA MOLECULES
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DOI：
10.1096/fasebj.7.1.7678559
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发表时间：
1993-01-01
期刊：
影响因子：
4.8
通讯作者：
BAILLEUL, B
中科院分区：
文献类型：
--
作者：
COCQUERELLE, C;MASCREZ, B;BAILLEUL, B
我们以前鉴定了新的人类ets-1转录本，其中外显子的正常顺序是颠倒的，并证明了尽管外显子的顺序与基因组DNA中的不同，但这些外显子的无序剪接是使用真正的剪接位点成对发生的（1）。在这里，我们确定了这些新的转录本的结构，表明它们对应于环状RNA分子，仅包含基因组顺序中的外显子。这些转录物是稳定的分子，定位于细胞的细胞质组分中。据我们所知，这是第一例在真核生物中从核前mRNA加工环状转录物。这种新型的转录本可能代表了基因表达的一个新方面，并为剪接机制提供了一些有趣的线索。
We previously identified novel human ets-1 transcripts in which the normal order of exons is inverted, and demonstrated that although the order of exons is different than in the genomic DNA, splicing of these exons out of order occurs in pairs using genuine splice sites (1). Here we determine the structure of these novel transcripts, showing that they correspond to circular RNA molecules containing only exons in genomic order. These transcripts are stable molecules, localized in the cytoplasmic component of the cells. To our knowledge, this is the first case of circular transcripts being processed from nuclear pre-mRNA in eukaryotes. This new type of transcript might represent a novel aspect of gene expression and hold some interesting clues about the splicing mechanism.