SINGLE-STRANDED DNA-PROTEIN BINDING IN THE PROCYCLIC ACIDIC REPETITIVE PROTEIN (PARP) PROMOTER OF TRYPANOSOMA-BRUCEI
SINGLE-STRANDED DNA-PROTEIN BINDING IN THE PROCYCLIC ACIDIC REPETITIVE PROTEIN (PARP) PROMOTER OF TRYPANOSOMA-BRUCEI
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DOI：
10.1016/0166-6851(94)90120-1
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发表时间：
1994-05-01
影响因子：
1.5
通讯作者：
VANDERPLOEG, LHT
中科院分区：
文献类型：
--
作者：
BROWN, SD;VANDERPLOEG, LHT
我们使用来自原生动物寄生虫布鲁氏锥虫的前环酸性重复蛋白（PARP）启动子的DNA进行了DNA-蛋白相互作用的凝胶阻滞分析。布鲁氏锥虫的PARP基因以抗a- amantin的方式转录，已经提出RNA聚合酶I而不是RNA聚合酶II转录PARP基因[11]。含有必需parp启动子区域的双链限制性片段仅与序列非特异性核因子结合，即使在提取物中存在特异性结合snRNA U2启动子双链DNA的蛋白因子。相比之下，单链dna结合蛋白以高亲和力、核苷酸序列和链特异性结合-69/-55元件和-37/-11元件的编码链和非编码链。
We performed gel retardation analyses of DNA-protein interactions using DNA from the procyclic acidic repetitive protein (PARP) promoter of the protozoan parasite Trypanosoma brucei. The PARP genes of Trypanosoma brucei are transcribed in an a-amanitin resistant manner, and it has been proposed that RNA polymerase I, rather than RNA polymerase II, transcribes the PARP genes [11]. Double-stranded restriction fragments containing the essential PARP-promoter regions bound only sequence-nonspecific nuclear factors, even though protein factors that bind specifically to double-stranded DNA from the snRNA U2 promoter were present in the extracts. In constrast, single-stranded DNA-binding proteins bound with high affinity, nucleotide-sequence and strand-specificity to the -69/-55 element and the coding and non-coding strands of the -37/-11 element.