Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques
Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques
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DOI：
10.1080/16583655.2019.1582460
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发表时间：
2019-01-01
影响因子：
3.3
通讯作者：
Kabbashi, Nassereldeen Ahmed
中科院分区：
文献类型：
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作者：
Alara, Oluwaseun Ruth;Abdurahman, Nour Hamid;Kabbashi, Nassereldeen Ahmed
采用索氏提取技术和微波辅助提取(MAE)技术，用乙醇提取杏仁叶中的活性成分。采用标准技术对乙醇提取物进行了植物化学分析。这一分析揭示了黄酮类、生物碱、类固醇、萜类、糖苷、单宁、酚、皂苷的存在，而不存在蒽醌类化合物。此外，气相色谱-质谱仪(GC-MS)分析结果表明，与索氏提取法相比，MAE提取的提取物中含有更多的植物化学物质，因此植物酚所占的比例更高。傅里叶变换红外光谱分析证实提取物中含有植物化学物质。并用DPPH法和ABTS法测定了提取物的IC50值。提取物的总酚含量、总黄酮含量与抗氧化活性之间存在明显的相关性。因此，本研究表明，利用微波辅助提取技术可以从杏仁叶中提取出具有天然抗氧化剂作用的高级酚类化合物。
The bioactive compounds in Vernonia amygdalina leaf were extracted using ethanol through Soxhlet and microwave-assisted extraction (MAE) techniques. The phytochemical analysis was carried out on the ethanolic extracts using the standard techniques. This analysis revealed the presence of flavonoids, alkaloids, steroids, terpenoids, glycosides, tannins, phenols, saponins, and the absence of anthraquinones. Furthermore, gas chromatography-mass spectroscopy (GC-MS) analysis results revealed the presence of more phytochemicals in the extract obtained through MAE compared to Soxhlet extraction technique, whereby phytol was present in a higher percentage. Fourier transform infrared spectroscopy analysis confirmed the presence of phytochemicals in the extracts. Moreover, the IC50 values of the extracts antioxidant activities were evaluated using DPPH and ABTS assays. There exists a clear correlation between total phenolic content, total flavonoid content and antioxidant activity of the extracts. Therefore, this study suggested that higher phenolic compounds responsible for natural antioxidant could be obtained from V. amygdalina leaf using the microwave-assisted extraction technique.