Ginsenoside Rg1 ameliorates the cognitive deficits in D-galactose and AlCl3-induced aging mice by restoring FGF2-Akt and BDNF-TrkB signaling axis to inhibit apoptosis
Ginsenoside Rg1 ameliorates the cognitive deficits in D-galactose and AlCl3-induced aging mice by restoring FGF2-Akt and BDNF-TrkB signaling axis to inhibit apoptosis
复制标题
人参皂苷 Rg1 通过恢复 FGF2-Akt 和 BDNF-TrkB 信号轴抑制细胞凋亡，改善 D-半乳糖和 AlCl3 诱导的衰老小鼠的认知缺陷
DOI：
10.7150/ijms.43979
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发表时间：
2020-01-01
影响因子：
3.6
通讯作者：
Qin, Xiao-Yan
中科院分区：
文献类型：
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作者：
Zhong, Si-Jia;Wang, Lin;Qin, Xiao-Yan
人参皂苷Rg1是人参的主要活性成分，具有神经保护、抗氧化和增强免疫系统的作用。因此，我们假设Rg1可能具有抗衰老作用，尽管其机制仍有待阐明。在本研究中，通过连续给药d -半乳糖和AlCl3建立化学诱导衰老小鼠。我们发现Rg1有效地改善了衰老小鼠的空间学习和记忆缺陷，这在退阶回避实验和Morris水迷宫实验中得到了证明。Rg1恢复衰老诱导的FGF2和BDNF的下降，重新激活海马和前额叶皮层的TrkB/Akt信号通路，抑制细胞凋亡，拮抗凋亡蛋白Bcl-2和促凋亡酶cleaved-Caspase3的表达。因此，这些结果确定了Rg1的抗衰老作用，而FGF2、BDNF及其相关信号通路可能是有希望的靶点。本研究结果可能为Rg1等民族产品抗衰老及衰老相关疾病的药理研究和饮食治疗作用提供新的途径。
Ginsenoside Rg1 is the main active ingredient of Panax ginseng with the activity of neuroprotective, antioxidant and strengthening the immune system. Therefore, we hypothesized that Rg1 may afford anti-aging effects although the mechanism remains to be elucidated. In this study, chemically induced aging mice were established by consecutive administration of D-galactose and AlCl3. We found that Rg1 effectively ameliorates spatial learning and memory deficits in aging mice demonstrated by their improved performance in step down avoidance tests and Morris water maze experiments. Rg1 restored aging-induced decline of FGF2 and BDNF, reactivated TrkB/Akt signaling pathways in the hippocampus and prefrontal cortex to inhibit apoptosis, for the expression of anti-apoptotic protein Bcl-2 and apoptosis promoting enzyme cleaved-Caspase3 were antagonistically restored. Therefore, these results established the anti-aging effects of Rg1, and FGF2, BDNF and associated signaling pathways might be promising targets. Our data may provide a new avenue to the pharmacological research and diet therapeutic role of ethnic products such as Rg1 in anti-aging and aging associated diseases.