The fucoxanthin-chlorophyll proteins from a chromophyte alga are part of a large multigene family: Structural and evolutionary relationships to other light harvesting antennae
The fucoxanthin-chlorophyll proteins from a chromophyte alga are part of a large multigene family: Structural and evolutionary relationships to other light harvesting antennae
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DOI：
10.1007/s004380050334
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发表时间：
1996-12-13
期刊：
影响因子：
--
通讯作者：
Green, BR
中科院分区：
文献类型：
--
作者：
Durnford, DG;Aebersold, R;Green, BR
褐藻黄质-叶绿素蛋白(FCP)基因编码210个氨基酸的多肽，与其他藻类以及陆生植物和绿藻的叶绿素a/b结合蛋白(CAB)相似。假定的传输序列具有类似于信号序列的特征。异西格玛FCP基因是一个庞大的多基因家族的一部分，该家族包括至少两个编码两个显著不同的多肽(Fcp1、Fcp2)的成员。将FCP序列与最近确定的豌豆LHC II复合体的三维结构进行比较，表明许多被认为参与叶绿素结合和形成络合物稳定离子相互作用的关键氨基酸都很保守。对捕光蛋白序列的系统发育分析表明，几个色藻门的FCP形成了一个独立于甲藻内膜蛋白-叶绿素蛋白(IPCPs)的自然类群。虽然FCP和CAB基因有一个共同的祖先，但在绿藻和陆生植物中CAB LHC I和LHC II序列分离之前，这些谱系就相互分化了。
A fucoxanthin-chlorophyll protein (FCP) cDNA from the raphidophyte Heterosigma carterae encodes a 210-amino acid polypeptide that has similarity to other FCPs and to the chlorophyll a/b-binding proteins (CABs) of terrestrial plants and green algae. The putative transit sequence has characteristics that resemble a signal sequence. The Heterosigma fcp genes are part of a large multigene family which includes members encoding at least two significantly different polypeptides (Fcp1, Fcp2). Comparison of the FCP sequences to the recently determined three-dimensional structure of the pea LHC II complex indicates that many of the key amino acids thought to participate in the binding of chlorophyll and the formation of complex-stabilizing ionic interactions are well conserved. Phylogenetic analyses of sequences of light-harvesting proteins shows that the FCPs of several chromophyte phyla form a natural group separate from the intrinisic peridinin-chlorophyll proteins (iPCPs) of the dinoflagellates. Although the FCP and CAB genes shared a common ancestor, these lineages diverged from each other prior to the separation of the CAB LHC I and LHC II sequences in the green algae and terrestrial plants.