Glutamate accumulation by human gliomas and meningiomas in tissue culture
Glutamate accumulation by human gliomas and meningiomas in tissue culture
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组织培养中人胶质瘤和脑膜瘤的谷氨酸积累
DOI：
10.1016/0006-8993(76)90311-5
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发表时间：
1976
期刊：
影响因子：
2.9
通讯作者：
P. Kornblith
中科院分区：
文献类型：
--
作者：
R. M. Stewart;R. Martuza;R. Baldessarini;P. Kornblith
研究了放射性谷氨酸在组织培养的人脑肿瘤细胞中的快速积累。人星形细胞瘤MGH-LM细胞系对谷氨酸摄取具有高亲和力（明显km = 20 μM）和低亲和力（明显km = 0.2mM）。高亲和力成分依赖于钠、温度和能量，在细胞生长的融合期（静止期）存在，而不是对数期。一项对其他几种人类脑肿瘤的融合单层培养的研究表明，谷氨酸的高亲和力摄取发生在8个恶性星形细胞瘤中的7个，并且似乎与恶性肿瘤的等级无关。在研究的3个视神经胶质瘤和5个脑膜瘤中，发现谷氨酸低但不高的亲和力摄取。这些研究表明，人类大脑中的神经胶质细胞在某些情况下可能具有类似于神经末梢的谷氨酸摄取特征。
The rapid accumulation of radioactive glutamate was studied in human brain tumor cells grown in tissue culture. The MGH-LM line of human astrocytoma demonstrated both a high (apparentKm= 20 μM) and a low (apparentKm= 0.2mM) affinity component for the uptake of glutamate. The high affinity component was sodium-, temperature-, and energy-dependent and was present during the confluent (stationary) but not the log phase of cell growth. A study of confluent monolayer cultures of several other human brain tumors revealed that a similar high affinity uptake of glutamate occurred in 7 out of 8 malignant astrocytomas and appeared to be independent of the grade of malignancy. In 3 optic gliomas and 5 meningiomas studied, low but not high affinity uptake of glutamate was found. These studies suggest that glial cells in the human brain may in some instances possess uptake characteristics for glutamate similar to those previously ascribed to nerve endings.