SERA FROM PATIENTS WITH CHRONIC LYME-DISEASE PROTECT MICE FROM LYME-BORRELIOSIS
SERA FROM PATIENTS WITH CHRONIC LYME-DISEASE PROTECT MICE FROM LYME-BORRELIOSIS
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DOI：
10.1093/infdis/169.3.568
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发表时间：
1994-03-01
影响因子：
6.4
通讯作者：
FLAVELL, RA
中科院分区：
文献类型：
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作者：
FIKRIG, E;BOCKENSTEDT, LK;FLAVELL, RA
来自不同阶段的莱姆病患者的血清用于被动免疫小鼠以对抗伯氏疏螺旋体攻击，以确定人类抗体是否可以保护动物免受感染。来自2例晚期莱姆病患者的血清，其中含有对B中蛋白质的强抗体反应性。包括针对外表面蛋白（Osps）A和B的抗体的伯氏菌裂解物在用B的小接种物（10（2））攻击后部分保护小鼠免受感染。burgdorferi。相对于对照小鼠（患者1血清，90%;患者2血清，74%），用来自这2名患者中任一名的血清免疫的小鼠发展出显著更少的疏螺旋体感染（患者1血清，5%;患者2血清，25%）。相反，来自2名缺乏对OspA和OspB反应的抗体的早期或晚期莱姆病患者的血清没有提供保护。免疫力似乎至少部分与对Osps的强烈体液反应的存在有关。这些结果表明，在长期感染期间，一些患者会产生免疫反应，可能部分防止B再次感染。burgdorferi。因此，尽管大多数患者在自然感染期间不会对Osp产生强烈的体液应答，但接种Osp可引起保护性免疫。
Sera from selected patients with Lyme disease in different stages were used to passively immunize mice against Borrelia burgdorferi challenge to determine if human antibodies could protect the animals from infection. Sera from 2 patients with late-stage Lyme disease that contained strong antibody reactivity to proteins in B. burgdorferi lysates, including antibodies to the outer surface proteins (Osps) A and B, partly protected mice from infection after challenge with a small inoculum (10(2)) of B. burgdorferi. Mice immunized with sera from either of these 2 patients developed significantly fewer infections from the borreliae (patient 1 serum, 5%; patient 2 serum, 25%) relative to control mice (patient 1 serum, 90%; patient 2 serum, 74%). In contrast, sera from 2 patients with early or late Lyme disease that lacked antibodies reactive to OspA and OspB did not confer protection. Immunity appeared to be related, at least in part, to the presence of a strong humoral response to the Osps. These results suggest that during prolonged infection, some patients develop an immune response that may be partly protective against reinfection with B. burgdorferi. Therefore, although most patients do not mount a strong humoral response to the Osps during natural infection, vaccination with an Osp may elicit protective immunity.