Evidence for autoregulation of cystathionine γ-synthase mRNA stability in Arabidopsis
Evidence for autoregulation of cystathionine γ-synthase mRNA stability in Arabidopsis
复制标题
DOI：
10.1126/science.286.5443.1371
复制
发表时间：
1999-11-12
期刊：
影响因子：
56.9
通讯作者：
Naito, S
中科院分区：
文献类型：
--
作者：
Chiba, Y;Ishikawa, M;Naito, S
信使RNA（mRNA）稳定性的控制是调节基因表达的重要机制。对过量积累可溶性甲硫氨酸（Met）的拟南芥突变体的分析表明，Met生物合成中的关键酶胱硫醚γ-合酶（CCS）的基因在mRNA稳定性水平上受到调控，用野生型-CCS基因的类型和突变形式表明，由CCS的第一外显子编码的氨基酸序列顺式作用，以使其自身的mRNA在由甲硫氨酸或其代谢产物。
Control of messenger RNA (mRNA) stability serves as an important mechanism, for regulating gene expression. Analysis of Arabidopsis mutants that overaccumulate soluble methionine (Met) revealed that the gene for cystathionine gamma-synthase (CCS), the key enzyme in Met biosynthesis, is regulated at the Level of mRNA stability, Transfection experiments with wild-type and mutant forms of the CCS gene suggest that an amino acid sequence encoded by the first exon of CCS acts in cis to destabilize its own mRNA in a process that is activated by Met or one of its metabolites.