Complete mitochondrial genome sequence of black mustard (Brassica nigra; BB) and comparison with Brassica oleracea (CC) and Brassica carinata (BBCC)
Complete mitochondrial genome sequence of black mustard (Brassica nigra; BB) and comparison with Brassica oleracea (CC) and Brassica carinata (BBCC)
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DOI：
10.1139/gen-2014-0165
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发表时间：
2014-11-01
期刊：
影响因子：
3.1
通讯作者：
Terachi, Toru
中科院分区：
文献类型：
--
作者：
Yamagishi, Hiroshi;Tanaka, Yoshiyuki;Terachi, Toru
芸苔属作物包括3个单基因组种和3个通过种间杂交产生的双二倍体种。到目前为止，这些物种中只有五种的线粒体基因组序列可用。对第六个物种黑芥（Brassica nigra，核基因组组成BB）的线粒体基因组进行了测序，并与甘蓝（Brassica oleracea，CC）和埃塞俄比亚芥（Brassica carinata，BBCC）的线粒体基因组进行了比较。基因组组装成一个232 145 bp的环状序列，略大于B。oleracea（219952 bp）。B的基因组。nigra含有33个蛋白质编码基因、3个rRNA基因和17个tRNA基因。cox 2 -2基因存在于B中。B中不存在甘蓝。黑鬼B. nigra和B.在carinata中，rps 3的第二个外显子显示出包括插入/缺失（indel）和核苷酸替换的差异。检测插入缺失的PCR测试揭示了rps 3和B的一个系中的种内变异。nigra扩增出预期大小为B的DNA片段。carinata。此外，B.这里测试的隆线产生了预期B大小的DNA片段。黑鬼结果表明，至少有两种B.黑质在B的母本中存在。carinata。
Crop species of Brassica (Brassicaceae) consist of three monogenomic species and three amphidiploid species resulting from interspecific hybridizations among them. Until now, mitochondrial genome sequences were available for only five of these species. We sequenced the mitochondrial genome of the sixth species, Brassica nigra (nuclear genome constitution BB), and compared it with those of Brassica oleracea (CC) and Brassica carinata (BBCC). The genome was assembled into a 232 145 bp circular sequence that is slightly larger than that of B. oleracea (219 952 bp). The genome of B. nigra contained 33 protein-coding genes, 3 rRNA genes, and 17 tRNA genes. The cox2-2 gene present in B. oleracea was absent in B. nigra. Although the nucleotide sequences of 52 genes were identical between B. nigra and B. carinata, the second exon of rps3 showed differences including an insertion/deletion (indel) and nucleotide substitutions. A PCR test to detect the indel revealed intraspecific variation in rps3, and in one line of B. nigra it amplified a DNA fragment of the size expected for B. carinata. In addition, the B. carinata lines tested here produced DNA fragments of the size expected for B. nigra. The results indicate that at least two mitotypes of B. nigra were present in the maternal parents of B. carinata.