The root-like chitosan nanofiber porous scaffold cross-linked by genipin with type I collagen and its osteoblast compatibility
The root-like chitosan nanofiber porous scaffold cross-linked by genipin with type I collagen and its osteoblast compatibility
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京尼平与I型胶原交联的根状壳聚糖纳米纤维多孔支架及其成骨细胞相容性
DOI：
10.1016/j.carbpol.2022.119255
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发表时间：
2022
影响因子：
11.2
通讯作者：
Xiaofeng Li
中科院分区：
文献类型：
--
作者：
Sihan Zhang;Guanglei Zhao;Wei Ma;Yanghui Song;Cheng Huang;Chong Xie;Kebing Chen;Xiaofeng Li
由于细胞外基质的致密结构，骨组织修复是困难的。为了解决这一问题，以京尼平（Gen）为交联剂，将根状壳聚糖（CSNF）与胶原（Col）交联，制备了具有细胞外基质样结构的多孔壳聚糖生物支架（CSNFS）。CSNFS的最佳制备条件为：CSNF：Col：Gen =1：1：0.1，在37 °C下交联48 h。CSNFS具有较高的孔隙率和足够的微米级孔，其BET数据显示存在大量的纳米级孔。在干态和湿态下，CSNFS的机械强度均高于壳聚糖支架。MC3T3细胞在CSNFS上生长良好，能在三维空间内长满支架，并在支架内贴壁分化良好。交联CSNFS具有良好的生物相容性，可作为骨组织工程的修复材料。
Bone tissue repair is difficult due to the dense structure of the extracellular matrix. To solve this problem, a porous chitosan nanofiber scaffold (CSNFS) with an extracellular matrix-like structure was prepared via a facile cross-linked reaction of root-like chitosan nanofiber (CSNF) and collagen (Col) by using genipin (Gen) as the cross-linker. The optimal preparation conditions of CSNFS is weight ratio of CSNF:Col:Gen =1:1:0.1, crosslinked 48 h under 37 °C. CSNFS shows high porosity with adequate micro-scale pores, and its BET data shows that there are a large number of nano-scale pores. The CSNFS mechanical strength is higher than that of the chitosan scaffold both in dry and wet state. MC3T3 cells grow well on CSNFS, can overgrow the scaffold in three-dimensional space, adhere and differentiate well within those nanofiber structure. The cross-linked CSNFS has good biocompatibility and can be used as a repair material for bone tissue engineering.