Reliability of a novel neurostimulation method to study involuntary muscle phenomena
Reliability of a novel neurostimulation method to study involuntary muscle phenomena
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DOI：
10.1002/mus.20903
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发表时间：
2008-01-01
期刊：
影响因子：
3.4
通讯作者：
De Grandis, Domenico
中科院分区：
文献类型：
--
作者：
Minetto, Marco Alessandro;Botter, Alberto;De Grandis, Domenico
涉及外周神经的疼痛电刺激的实验方法表明存在能够诱导痉挛的最小刺激频率，称为“阈值频率”（TF）。我们的目的是测试一种替代方法，以诱导肌束震颤和痉挛电。在3天内对19名志愿者进行两次拇展肌电刺激：将频率递增的150个双相方脉冲（持续时间152 μ s）的刺激串（电流强度比最大值高30%;第一次试验的频率为4 pps;试验之间的恢复时间为1 min）传递到主要肌肉运动点，直到出现痉挛。一旦痉挛被诱导的协议，30分钟后重复。为了验证通过肌电图发生痉挛，表面电极阵列被放置在运动点和远端肌腱。环境温度和皮肤温度在所有阶段保持恒定。所有受试者均出现肌束震颤和痉挛。我们观察到以下TF的中位数（四分位距）值：第1天（第1阶段），13（6）pps;第1天（第2阶段），16（4）pps;第2天（第1阶段），16（6）pps;第2天（第2阶段），18（6）pps;第3天（第1阶段），17（4）pps;第3天（第2阶段），18（8）pps。TF会话间组内相关系数分别为0.82，0.92和0.90的第1，2和3天。TF日间组内相关系数为0.85。没有疼痛，由于刺激和TF的可靠性证明支持使用我们的方法研究不随意肌现象。
Experimental methods involving painful electrical stimulation of a peripheral nerve showed the existence of a minimum stimulation frequency capable of inducing cramp, termed "threshold frequency" (TF). Our aim was to test an alternative method to induce fasciculations and cramps electrically. Two daily sessions of electrical stimulation of the abductor hallucis muscle were performed in 19 volunteers on 3 days: stimulation trains of 150 monophasic square pulses (duration 152 mu s) of increasing frequency (current intensity 30% higher than maximal; frequency of the first trial, 4 pps; recovery between trials, 1 min) were delivered to the main muscle motor point until a cramp developed. Once a cramp was induced the protocol was repeated after 30 min. To verify by electromyography that cramp occurred, a surface electrode array was placed between the motor point and the distal tendon. Ambient and skin temperature were kept constant in all sessions. Fasciculations and cramps were elicited in all subjects. We observed the following median (interquartile range) values of TF: day 1 (session 1), 13 (6) pps; day 1 (session 2), 16 (4) pps; day 2 (session 1), 16 (6) pps; day 2 (session 2), 18 (6) pps; day 3 (session 1), 17 (4) pps; day 3 (session 2), 18 (8) pps. TF intersession intraclass correlation coefficients were 0.82, 0.92, and 0.90 for days 1, 2, and 3, respectively. TF interday intraclass correlation coefficient was 0.85. The absence of pain due to the stimulation and the demonstration of TF reliability support the use of our method for the study of involuntary muscle phenomena.