Mulberry leaf ameliorates the expression profile of adipocytokines by inhibiting oxidative stress in white adipose tissue in db/db mice
Mulberry leaf ameliorates the expression profile of adipocytokines by inhibiting oxidative stress in white adipose tissue in db/db mice
复制标题
DOI：
10.1016/j.atherosclerosis.2008.10.021
复制
发表时间：
2009-06-01
期刊：
影响因子：
5.3
通讯作者：
Yokode, Masayuki
中科院分区：
文献类型：
--
作者：
Sugimoto, Masayuki;Arai, Hidenori;Yokode, Masayuki
已有研究表明，桑树(Morus alba L.)Leaf(ML)改善apoE(-/-)小鼠的动脉粥样硬化。尽管脂肪细胞因子失调是动脉粥样硬化性心血管疾病的重要危险因素，但ML对与脂肪细胞因子失调和炎症相关的代谢紊乱的影响尚未被研究。因此，我们研究了ML在代谢紊乱中的作用，并探讨了ML改善db/db小鼠代谢紊乱的机制。我们用ML和/或吡格列酮治疗db/db小鼠12周，发现ML降低血糖和血浆甘油三酯。与吡格列酮相比，ML和吡格列酮联合作用具有相加效应。此外，他们的共同治疗减轻了在吡格列酮治疗下观察到的体重增加。治疗后白色脂肪组织脂联素表达增加，肿瘤坏死因子-α、单核细胞趋化蛋白-1和巨噬细胞标志物表达降低。此外，ML还能降低WAT和肝脏中的过氧化脂质和NADPH氧化酶亚基的表达。他们的共同治疗加强了这些影响。因此，ML至少部分通过抑制db/db小鼠WAT中的氧化应激来改善脂肪细胞因子的失调，ML可能是治疗代谢综合征以及减少吡格列酮不良反应的药物的基础。(C)2008爱思唯尔爱尔兰有限公司。保留所有权利。
Previous study showed that mulberry (Morus Alba L.) leaf (ML) ameliorates atherosclerosis in apoE(-/-) mice. Although the adipocytokine dysregulation is an important risk factor for atherosclerotic cardiovascular disease, the effect of ML on metabolic disorders related to adipocytokine dysregulation and inflammation has not been studied. Therefore, we studied the effects of ML in metabolic disorders and examined the mechanisms by which ML ameliorates metabolic disorders in db/db mice. We treated db/db mice with ML, pioglitazone, or both for 12 weeks and found that ML decreased blood glucose and plasma triglyceride. Co-treatment with ML and pioglitazone showed additive effects compared with pioglitazone. Moreover, their co-treatment attenuated the body weight increase observed under the pioglitazone treatment. ML treatment also increased the expression of adiponectin, and decreased the expression of TNF-alpha, MCP-1, and macrophage markers in white adipose tissue (WAT). Furthermore, ML decreased lipid peroxides and the expression of NADPH oxidase subunits in WAT and liver. Their co-treatment enhanced these effects. Thus, ML ameliorates adipocytokine dysregulation at least in part through inhibiting oxidative stress in WAT of db/db mice, and that ML may be a basis for a pharmaceutical for the treatment of the metabolic syndrome as well as reducing adverse effects of pioglitazone. (C) 2008 Elsevier Ireland Ltd. All rights reserved.