TUBULAR LYSOSOME MORPHOLOGY AND DISTRIBUTION WITHIN MACROPHAGES DEPEND ON THE INTEGRITY OF CYTOPLASMIC MICROTUBULES
TUBULAR LYSOSOME MORPHOLOGY AND DISTRIBUTION WITHIN MACROPHAGES DEPEND ON THE INTEGRITY OF CYTOPLASMIC MICROTUBULES
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DOI：
10.1073/pnas.84.7.1921
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发表时间：
1987-04-01
影响因子：
11.1
通讯作者：
SILVERSTEIN, SC
中科院分区：
文献类型：
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作者：
SWANSON, J;BUSHNELL, A;SILVERSTEIN, SC
荧光染料荧光黄标记的胞饮作用在几种细胞类型中延长，膜结合的管状细胞器，包括培养的人单核细胞，硫代乙酸酯诱导的小鼠腹膜巨噬细胞，以及巨噬细胞样细胞系J774.2。酸性磷酸酶组织化学和组织蛋白酶L免疫荧光定位表明，这些小管结构为溶酶体。佛波酯(10 ng/ml)处理后，小管溶酶体的丰度明显增加。当用微量过氧化物酶的胞吞作用标记并经电子显微镜组织化学检查时，管状溶酶体的外径为apprxeq。75 nm，长度几微米：它们从细胞的S中心球放射出来，与细胞质微管和中间丝排成一列。佛波酯处理的巨噬细胞在4℃孵育。C或在37℃含有5微米秋水仙碱或诺可唑的培养基中。C导致微管解体和管状溶酶体碎裂。文化回归到37度。在10-20分钟内，除去培养液中的诺康唑会导致微管的重新组装和管状溶酶体的重新出现。我们得出的结论是，微管对于维持管状溶酶体的形态是必不可少的，在巨噬细胞中，溶酶体室的很大比例包含在这些管状结构中。
Pinocytosis of the fluorescent dye lucifer yellow labels elongated, membrane-bound tubular organelles in several cell types, including cultured human monocytes, thioglycolate-elicited mouse peritoneal macrophages, and the macrophage-like cell line J774.2. These tubular structures can be identified as lysosomes by acid phosphatase histochemistry and immunofluorescence localization of cathepsin L. The abundance of tubular lysosomes is markedly increased by treatment with phorbol 2-myristate 13-acetate (10 ng/ml). When labeled by pinocytosis of microperoxidase and examined by electron microscopic histochemistry, the tubular lysosomes have an outside diameter of .apprxeq. 75 nm and a length of several micrometers: they radiate from the cell''s centrosphere in aligment with cytoplasmic microtubules and intermediate filaments. Incubation of phorbol myristate acetate-treated macrophages at 4.degree. C or in medium containing 5 .mu.M colchicine or nocodazole at 37.degree. C leads to disassembly of microtubules and fragmentation of the tubular lysosomes. Return of the cultures to 37.degree. C removal of nocodazole from the medium leads to reassembly of microtubules and the reappearance of tubular lysosomes within 10-20 min. We conclude that microtubules are essential for the maintenance of tubular lysosome morphology and that, in macrophages, a significant proportion of the lysosomal compartment is contained within these tubular structures.