Dosage effect on uropathogenic Escherichia coli anti-adhesion activity in urine following consumption of cranberry powder standardized for proanthocyanidin content: a multicentric randomized double blind study.
Dosage effect on uropathogenic Escherichia coli anti-adhesion activity in urine following consumption of cranberry powder standardized for proanthocyanidin content: a multicentric randomized double blind study.
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DOI：
10.1186/1471-2334-10-94
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发表时间：
2010-04-14
影响因子：
3.7
通讯作者：
Lavigne JP
中科院分区：
文献类型：
--
作者：
Howell AB;Botto H;Combescure C;Blanc-Potard AB;Gausa L;Matsumoto T;Tenke P;Sotto A;Lavigne JP
摄入蔓越莓（Vaccinium macrocarpon Ait.）传统上用于预防尿路感染。蔓越莓中的原花青素 (PAC)，特别是 A 型连接，被认为是主要 P 菌毛状大肠杆菌与尿上皮细胞粘附的重要抑制剂。需要进行额外的实验来研究尿液样本在更广泛的时间内的持久性，以确定每天最有效的剂量，并确定是否在不同来源的志愿者中检测到蔓越莓的尿液抗粘连作用。两项独立的生物测定（甘露糖抗性血凝测定和原创的新型人类 T24 上皮细胞系测定）评估了在一项随机、双盲与安慰剂研究中从日本、匈牙利、西班牙和法国收集的 32 名志愿者的尿液样本的离体尿液细菌抗粘附活性。使用体内秀丽隐杆线虫模型来评估蔓越莓方案对大肠杆菌菌株毒力的影响。结果表明，与安慰剂相比，从食用蔓越莓粉的志愿者收集的尿液样本中具有显着的细菌抗粘附活性（p < 0.001）。这种抑制作用明显呈剂量依赖性，持续时间较长（72 mg PAC 直至 24 小时），并且随着每种蔓越莓粉方案中消耗的 PAC 当量量而增加。体内秀丽隐杆线虫模型表明，蔓越莓具有对抗细菌毒力的作用：服用蔓越莓胶囊的患者尿液样本中出现生长后，大肠杆菌菌株杀死蠕虫的能力降低。这种作用对于 72 mg PAC 的治疗方案尤为重要。每天服用含有 72 毫克 PAC 的 PAC 标准化蔓越莓粉，可以在一定程度上防止泌尿道细菌粘附和毒力。这种作用可以提供夜间保护。
Ingestion of cranberry (Vaccinium macrocarpon Ait.) has traditionally been utilized for prevention of urinary tract infections. The proanthocyanidins (PACs) in cranberry, in particular the A-type linkages have been implicated as important inhibitors of primarily P-fimbriated E. coli adhesion to uroepithelial cells. Additional experiments were required to investigate the persistence in urine samples over a broader time period, to determine the most effective dose per day and to determine if the urinary anti-adhesion effect following cranberry is detected within volunteers of different origins. Two separate bioassays (a mannose-resistant hemagglutination assay and an original new human T24 epithelial cell-line assay) have assessed the ex-vivo urinary bacterial anti-adhesion activity on urines samples collected from 32 volunteers from Japan, Hungary, Spain and France in a randomized, double-blind versus placebo study. An in vivo Caenorhabditis elegans model was used to evaluate the influence of cranberry regimen on the virulence of E. coli strain. The results indicated a significant bacterial anti-adhesion activity in urine samples collected from volunteers that consumed cranberry powder compared to placebo (p < 0.001). This inhibition was clearly dose-dependent, prolonged (until 24 h with 72 mg of PAC) and increasing with the amount of PAC equivalents consumed in each cranberry powder regimen. An in vivo Caenorhabditis elegans model showed that cranberry acted against bacterial virulence: E. coli strain presented a reduced ability to kill worms after a growth in urines samples of patients who took cranberry capsules. This effect is particularly important with the regimen of 72 mg of PAC. Administration of PAC-standardized cranberry powder at dosages containing 72 mg of PAC per day may offer some protection against bacterial adhesion and virulence in the urinary tract. This effect may offer a nyctohemeral protection.