Expression of Human MCM6 and DNA Topo II α in craniopharyngiomas and its correlation with recurrence of the tumor
Expression of Human MCM6 and DNA Topo II α in craniopharyngiomas and its correlation with recurrence of the tumor
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DOI：
10.1007/s11060-006-9284-0
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发表时间：
2007-04
影响因子：
3.9
通讯作者：
Jianguo Xu;Si-zhong Zhang;C. You;Si-qing Huang;Bo-wen Cai;Xiaojie Wang
中科院分区：
文献类型：
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作者：
Jianguo Xu;Si-zhong Zhang;C. You;Si-qing Huang;Bo-wen Cai;Xiaojie Wang
颅咽管瘤切除后经常复发，导致受影响患者的预后不佳。仍然缺乏预测肿瘤行为的可靠标准。有人提出，肿瘤细胞的增殖潜力对于复发是必要的。本研究评估了 32 名金刚上皮瘤 (AE) 患者和 31 名鳞状乳头状瘤 (SP) 患者的细胞增殖活性，这可能与复发有关。为此，收集了原发性和复发性肿瘤的组织标本，并通过定量显微镜对肿瘤切片中微染色体维持蛋白 6 (MCM6) 和 DNA 拓扑异构酶 II α (DNA Topo II α) 的表达进行了免疫组织化学分析。结果发现，AE中的MCM6标记指数（LI）显着高于SP，并且在两种亚型的原发肿瘤中，复发肿瘤的LI均高于未复发肿瘤。在所研究的颅咽管瘤中，MCM6 LI 和 DNA Topo II α LI 之间存在很强的线性正相关性 (r= 0.713；p= 0.000)。 20例复发性颅咽管瘤的中位MCM6 LI（31.49%）与原发肿瘤（29.65%）无显着差异。 AE 中肿瘤复发的长期风险高于 SP，并且与 MCM6 和 DNA Topo II α 表达相关。
Craniopharyngioma often recurs after resection resulting in poor outcome for the affected patients. The reliable criteria for predicting the tumor behavior are still lacking. It has been suggested that proliferative potential of the tumor cells is necessary for recurrence. Present study evaluated the cell multiplication activity, which is possibly related to relapse in 32 patients with adamantine epithelioma (AE) and 31 patients with squamous papillary tumor (SP). For this tissue specimens from their primary and recurring tumors were collected and immunohistochemical analysis of the expression of minichromosome maintenance protein 6 (MCM6) and DNA topoisomerase II αlpha (DNA Topo II α) in the tumor sections was performed by quantitative microscopy. It was found that the MCM6 label index (LI) was significantly higher in AE than that of SP, and in the primary tumors of both subtypes the LIs of tumors with recurrence were higher than those without recurrence. There was a strong linear positive correlation between MCM6 LI and DNA Topo II α LI (r= 0.713;p= 0.000) in the craniopharyngiomas studied. The median MCM6 LI of the total 20 recurrent craniopharyngiomas (31.49%) was not significantly different from that of their primary tumors (29.65%). The long term risk of tumor recurrence is higher in AE than SP and it is associated with MCM6 and DNA Topo II α expression.