Genetic content of wild-type human cytomegalovirus
Genetic content of wild-type human cytomegalovirus
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DOI：
10.1099/vir.0.79888-0
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发表时间：
2004-05-01
影响因子：
3.8
通讯作者：
Davison, AJ
中科院分区：
文献类型：
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作者：
Dolan, A;Cunningham, C;Davison, AJ
通过对一株人巨细胞病毒低传代株(Merlin)的235645bp基因组测序，研究了野生型人巨细胞病毒的基因含量。基因组的大部分区域(基因RL1-UL11、UL105-UL112和UL120-UL150)也在其他几个菌株中进行了测序，其中包括两个未在细胞培养中传代的菌株。利用已公布的高传代菌株(AD169)的基因组序列进行的比较分析表明，Merlin准确地反映了165个基因的野生型互补，除了截短UL128基因的单核苷酸替换外，没有明显的突变。一个相当大的基因亚集在不同菌株之间表现出异常高的变异，并包括许多但不是所有编码已知或预测分泌或膜相关蛋白的基因。与未传代的菌株相比，所有分析的传代菌株在可能影响细胞趋向性的两组基因中的一组或两组中都有明显的致残突变。另一个含有RL11糖蛋白基因家族成员RL5A、RL13和UL9。支持UL105和UL111A之间区域缺乏蛋白质编码潜力的情况也得到了加强。
The genetic content of wild-type human cytomegalovirus was investigated by sequencing the 235 645 bp genome of a low passage strain (Merlin). Substantial regions of the genome (genes RL1-UL11, UL105-UL112 and UL120-UL150) were also sequenced in several other strains, including two that had not been passaged in cell culture. Comparative analyses, which employed the published genome sequence of a high passage strain (AD169), indicated that Merlin accurately reflects the wild-type complement of 165 genes, containing no obvious mutations other than a single nucleotide substitution that truncates gene UL128. A sizeable subset of genes exhibits unusually high variation between strains, and comprises many, but not all, of those that encode proteins known or predicted to be secreted or membrane-associated. In contrast to unpassaged strains, all of the passaged strains analysed have visibly disabling mutations in one or both of two groups of genes that may influence cell tropism. One comprises UL128, UL130 and UL131A, which putatively encode secreted proteins, and the other contains RL5A, RL13 and UL9, which are members of the RL11 glycoprotein gene family. The case in support of a lack of protein-coding potential in the region between UL105 and UL111 A was also strengthened.