ORIGIN, DIFFERENTIATION AND RENEWAL OF 4 MAIN EPITHELIAL-CELL TYPES IN MOUSE SMALL INTESTINE .2. MUCOUS CELLS
ORIGIN, DIFFERENTIATION AND RENEWAL OF 4 MAIN EPITHELIAL-CELL TYPES IN MOUSE SMALL INTESTINE .2. MUCOUS CELLS
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DOI：
10.1002/aja.1001410404
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发表时间：
1974-01-01
影响因子：
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通讯作者：
CHENG, H
中科院分区：
文献类型：
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作者：
CHENG, H
在单次注射或连续输注 3 H-胸苷后不同时间处死的小鼠中，借助放射自拍照，在光学和电子显微镜下研究十二指肠、空肠和回肠的粘液细胞群。粘液细胞的特征是粘液球和粗面内质网池扩张。可以识别粘液细胞的两个亚群，一个称为普通细胞，另一个称为颗粒细胞。颗粒状粘液细胞与普通粘液细胞的不同之处在于粘液球内存在嵌入的小而致密的颗粒。每个亚群又分为含有少量粘液球的未成熟少粘细胞和含有大量粘液球的成熟杯状细胞。普通和颗粒状少粘细胞仅存在于隐窝中，主要位于隐窝中下部，而相应的两种类型的杯状细胞存在于隐窝上部和绒毛下部。在绒毛上部仅观察到常见的粘液细胞。两种类型的少粘细胞（但杯状细胞除外）具有摄取3H-胸苷并分裂的能力。电子显微镜放射自显影表明，当少粘细胞向上迁移时，它们转变为杯状细胞。后者随后迁移至绒毛上皮。就颗粒状粘液细胞而言，这种迁移伴随着特征性致密颗粒的逐渐丧失，使得到达绒毛上部的颗粒状杯状细胞变成普通杯状细胞。绒毛上皮中的杯状细胞，无论其来源如何，都会上升到绒毛尖端，并在挤压区中丢失。普通粘液细胞的周转时间约为三天，柱状细胞则相反。颗粒状粘液细胞的细胞则稍短一些。在这两种情况下，少粘液细胞的分裂仅产生大约一半的粘液细胞。因此，另一半必须来自寡粘细胞以外的前体细胞。由于一些隐窝基底柱状细胞含有奇怪的粘液球，因此怀疑它们是两种类型寡粘液细胞的前体，并通过它们成为整个粘液细胞群的前体。
The mucous cell population of duodenum, jejunum and ileum was investigated in the light and electron microscopes with the help of radioautography in mice sacrificed at various times after single injection or continuous infusion of3H‐thymidine.Mucous cells are characterized by globules of mucus and by dilated cisternae of rough endoplasmic reticulum. Two subgroups of mucous cells, one calledcommonand the othergranular, may be identified. The granular mucous cells differ from the common ones by the presence of small dense granules embedded within the mucous globules. Each subgroup is further divided into immatureoligomucous cellscontaining few mucous globules, and maturegoblet cellswith a large accumulation of mucous globules.Common and granular oligomucous cells are found exclusively in the crypt, mainly within the lower mid‐crypt, whereas the corresponding two types of goblet cells are present in the upper part of the crypts and in the lower part of the villi. Only common mucous cells are observed in the upper part of the villi.The two types of oligomucous cells, but not goblet cells, have the ability to take up3H‐thymidine and divide. Electron microscopic radioautography demonstrates that, as oligomucous cells migrate upwards, they transform into goblet cells. The latter then migrate to the villus epithelium.In the case of granular mucous cells, this migration is associated with a gradual loss of the characteristic dense granules, so that the granular goblet cells reaching the upper part of the villi become common goblet cells. The goblet cells in the villus epithelium, regardless of their origin, ascend towards the villus tips where they are lost through the extrusion zones.The turnover time of common mucous cells is about three days, as for columnar cells; and that of granular mucous cells, somewhat shorter. In both cases, the divisions of oligomucous cells account only for the production of about half the mucous cells present. Hence, the other half must be derived from precursors other than oligomucous cells. Since a few crypt‐base columnar cells contain the odd mucous globule, they are suspected of being the precursors of the two types of oligomucous cells and, through them, of the entire mucous cell population.