Shape transition from elliptical to cylindrical membrane tubes induced by chiral crescent-shaped protein rods
Shape transition from elliptical to cylindrical membrane tubes induced by chiral crescent-shaped protein rods
复制标题
DOI：
10.1038/s41598-019-48102-7
复制
发表时间：
2018-07
影响因子：
4.6
通讯作者：
H. Noguchi
中科院分区：
文献类型：
--
作者：
H. Noguchi
蛋白质通常在生物膜上形成手性组装结构。然而，手性在与非手性膜相互作用中的作用还知之甚少。在这里，我们使用无网格膜模拟，报道了新月形蛋白质棒的手性如何改变它们的组装和管状。无手性棒通过稳定椭圆的边缘将膜管变形为椭圆形。相反，手性棒形成了一个螺旋组件，除了椭圆管外，它还产生了一个半径恒定的圆柱形膜管。这种螺旋组装可以通过蛋白质棒之间的直接侧到侧吸引来进一步稳定。手性也促进了从扁平的膜上的管状生长。这些结果与Bin/双体蛋白/RVS(BAR)超家族蛋白诱导的膜小管半径恒定的实验结果一致。
Proteins often form chiral assembly structures on a biomembrane. However, the role of the chirality in the interaction with an achiral membrane is poorly understood. Here, we report how chirality of crescent-shaped protein rods changes their assembly and tubulation using meshless membrane simulations. The achiral rods deformed the membrane tube into an elliptical shape by stabilizing the edges of the ellipse. In contrast, the chiral rods formed a helical assembly that generated a cylindrical membrane tube with a constant radius in addition to the elliptical tube. This helical assembly could be further stabilized by the direct side-to-side attraction between the protein rods. The chirality also promotes the tubulation from a flat membrane. These results agree with experimental findings of the constant radius of membrane tubules induced by the Bin/Amphiphysin/Rvs (BAR) superfamily proteins.