Broad Tissue and Cell Tropism of Avian Bornavirus in Parrots with Proventricular Dilatation Disease
Broad Tissue and Cell Tropism of Avian Bornavirus in Parrots with Proventricular Dilatation Disease
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DOI：
10.1128/jvi.00133-09
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发表时间：
2009-06-01
影响因子：
5.4
通讯作者：
Staeheli, Peter
中科院分区：
文献类型：
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作者：
Rinder, Monika;Ackermann, Andreas;Staeheli, Peter
禽博尔纳病毒（Avian Bornaviruses，ABV）是近年来在北美和以色列发现的一种新的博尔纳病毒属。我们在这里表明，密切相关的病毒也存在于圈养的欧洲鹦鹉的各种物种与PDD。我们在临床病禽中鉴定的六种ABV毒株是先前定义的ABV基因型2和4的新成员。这两种基因型的病毒很容易建立持续的，noncytolytic感染鹌鹑和鸡细胞系，但没有在培养的哺乳动物细胞中的经典博尔纳病病毒株复制非常有效地增长。ABV抗原存在于细胞质和细胞核中，表明ABV的核复制。禽和哺乳动物的博尔纳病毒的基因组结构是高度保守的，除了ABV在编码病毒蛋白X和P的双顺反子mRNA的5 '非编码区中缺乏独特的控制元件。逆转录-PCR分析表明，在许多（如果不是全部）患有PDD的鸟类器官中存在病毒。在病禽的粪便中也发现了病毒核酸，表明病毒通过粪便-口鼻途径传播。免疫组织化学分析显示，禽流感病毒感染的器官与PDD不仅限于神经系统的细胞。因此，ABV表现出广泛的组织和细胞嗜性，这与经典的博尔纳病病毒明显不同。
Avian bornaviruses (ABV), representing a new genus within the family Bornaviridae, were recently discovered in parrots from North America and Israel with proventricular dilatation disease (PDD). We show here that closely related viruses are also present in captive European parrots of various species with PDD. The six ABV strains that we identified in clinically diseased birds are new members of the previously defined ABV genotypes 2 and 4. Viruses of both genotypes readily established persistent, noncytolytic infections in quail and chicken cell lines but did not grow in cultured mammalian cells in which classical Borna disease virus strains replicate very efficiently. ABV antigens were present in both the cytoplasm and nucleus of infected cells, suggesting nuclear replication of ABV. The genome organization of avian and mammalian bornaviruses is highly conserved except that ABV lacks a distinct control element in the 5' noncoding region of the bicistronic mRNA encoding the viral proteins X and P. Reverse transcription-PCR analysis demonstrated the presence of virus in many, if not all, organs of birds with PDD. Viral nucleic acid was also found in feces of diseased birds, suggesting virus transmission by the fecal-oronasal route. Immunohistochemical analysis of organs from birds with PDD revealed that infection with ABV is not restricted to cells of the nervous system. Thus, ABV exhibits a broad tissue and cell tropism that is strikingly different from classical Borna disease virus.