Materials composed of the D rosophila Hox protein Ultrabithorax are biocompatible and nonimmunogenic: UBX Materials are Biocompatible and Non-Immunogenic
Materials composed of the D rosophila Hox protein Ultrabithorax are biocompatible and nonimmunogenic: UBX Materials are Biocompatible and Non-Immunogenic
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由果蝇 Hox 蛋白 Ultrabithorax 组成的材料具有生物相容性和非免疫原性： UBX 材料具有生物相容性和非免疫原性
DOI：
10.1002/jbm.a.35295
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发表时间：
2015
影响因子：
4.9
通讯作者：
Bondos, Sarah E.
中科院分区：
文献类型：
--
作者：
Patterson, Jan L.;Arenas-Gamboa, Angela M.;Wang, Ting-Yi;Hsiao, Hao-Ching;Howell, David W.;Pellois, Jean-Philippe;Rice-Ficht, Allison;Bondos, Sarah E.
虽然果蝇黑腹宿主蛋白Ubx的活体功能是调节转录，但在体外Ubx分级自组装形成纳米级到宏观级的材料。UBX材料的形态、机械性能和功能(通过蛋白质嵌合体)都很容易设计。UBX材料也与培养中的细胞兼容。这些特性使得UBX作为一种潜在的组织工程支架很有吸引力，但要用作这种支架，它们必须是生物相容的和非免疫原性的。在这项研究中，我们评估UBX材料是否适合活体应用。当Ubx纤维被植入小鼠体内时，几乎没有免疫细胞被吸引到植入区域。植入Ubx的小鼠的血清中几乎没有能够识别Ubx的抗体。此外，与巨噬细胞体外培养的UBX纤维不能溶解或激活巨噬细胞，通过肿瘤坏死因子-α和NO的分泌来检测。最后，UBX纤维在与人类红细胞孵育时不会引起溶血。观察到的最小影响与在体内成功使用的生物材料所产生的影响相当。我们得出结论：UBX材料具有生物相容性和非免疫原性。©2014 Wiley期刊，Inc.生物材料资源A部分：103A：1546-1553,2015。
Although thein vivofunction of theDrosophila melanogasterHox protein Ultrabithorax (Ubx) is to regulate transcription,in vitroUbx hierarchically self‐assembles to form nanoscale to macroscale materials. The morphology, mechanical properties, and functionality (via protein chimeras) of Ubx materials are all easily engineered. Ubx materials are also compatible with cells in culture. These properties make Ubx attractive as a potential tissue engineering scaffold, but to be used as such they must be biocompatible and nonimmunogenic. In this study, we assess whether Ubx materials are suitable forin vivoapplications. When implanted into mice, Ubx fibers attracted few immune cells to the implant area. Sera from mice implanted with Ubx contain little to no antibodies capable of recognizing Ubx. Furthermore, Ubx fibers cultured with macrophagesin vitrodid not lyse or activate the macrophages, as measured by TNF‐αand NO secretion. Finally, Ubx fibers do not cause hemolysis when incubated with human red blood cells. The minimal effects observed are comparable with those induced by biomaterials used successfullyin vivo. We conclude Ubx materials are biocompatible and nonimmunogenic. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1546–1553, 2015.