Low mitochondrial DNA diversity of Japanese Polled and Kuchinoshima feral cattle
Low mitochondrial DNA diversity of Japanese Polled and Kuchinoshima feral cattle
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DOI：
10.1111/asj.12716
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发表时间：
2017-05-01
影响因子：
2
通讯作者：
Sasazaki, Shinji
中科院分区：
文献类型：
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作者：
Mannen, Hideyuki;Yonesaka, Riku;Sasazaki, Shinji
本研究旨在评估日本无角野牛和库欣岛野牛的线粒体遗传多样性和结构，这两头野牛维持在较小的种群。我们测定了两个牛群体的线粒体DNA(MtDNA)置换环(D-loop)序列，并结合之前发表的东北亚牛群体数据进行了分析。我们的研究结果表明，日本本地牛具有亚洲特有的mtDNA单倍群T4，频率较高(0.43-0.81)。这排除了库奇诺岛牛(32头动物)，它们只有一个属于单倍组T3的mtDNA单倍型。日本调查显示，线粒体DNA的平均序列差异度(0.0020)低于其他和育品种(0.0036-0.0047)。日本民调一直维持在山口的有限区域内，目前人口规模不到200人。因此，日本受访群体线粒体DNA的低多样性可以用过去30年来群体规模的减少来解释。我们发现日本角牛和库奇诺岛牛的mtDNA多样性都很低。本研究获得的遗传信息将有助于维持这些群体，并有助于了解日本本地牛的起源。
This study aims to estimate the mitochondrial genetic diversity and structure of Japanese Polled and Kuchinoshima feral cattle, which are maintained in small populations. We determined the mitochondrial DMA (mtDNA) displacement loop (D-loop) sequences for both cattle populations and analyzed these in conjunction with previously published data from Northeast Asian cattle populations. Our findings showed that Japanese native cattle have a predominant, Asian-specific mtDNA haplogroup T4 with high frequencies (0.43-0.81). This excluded Kuchinoshima cattle (32 animals), which had only one mtDNA haplotype belonging to the haplogroup T3. Japanese Polled showed relatively lower mtDNA diversity in the average sequence divergence (0.0020) than other Wagyu breeds (0.0036-0.0047). Japanese Polled have been maintained in a limited area of Yamaguchi, and the population size is now less than 200. Therefore, low mtDNA diversity in the Japanese Polled could be explained by the decreasing population size in the last three decades. We found low mtDNA diversity in both Japanese Polled and Kuchinoshima cattle. The genetic information obtained in this study will be useful for maintaining these populations and for understanding the origin of Japanese native cattle.