Quantitative proteomic reveals the dynamic of protein profile during final oocyte maturation in zebrafish
Quantitative proteomic reveals the dynamic of protein profile during final oocyte maturation in zebrafish
复制标题
定量蛋白质组学揭示斑马鱼最终卵母细胞成熟过程中蛋白质谱的动态
DOI：
10.1016/j.bbrc.2017.06.093
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发表时间：
2017-08-26
影响因子：
3.1
通讯作者：
Chen, Aqin
中科院分区：
文献类型：
--
作者：
Ge, Chunmei;Lu, Weiqun;Chen, Aqin
斑马鱼（Danio rerio）卵母细胞最终成熟的分子机制仍然知之甚少。本研究旨在采用iTRAQ方法对斑马鱼卵母细胞成熟过程中的蛋白质组进行全面表征，并比较排卵前完全发育的未成熟卵母细胞和成熟卵母细胞之间的差异。共鉴定了1568个蛋白质，这是迄今为止最大的斑马鱼分离卵母细胞蛋白质组数据集。差异表达分析显示，有190个蛋白质在未成熟卵母细胞和成熟卵母细胞中发生了显著变化，其中136个蛋白质在成熟卵母细胞中表达上调，54个蛋白质在成熟卵母细胞中表达下调。功能分析表明，这些差异蛋白主要参与雌激素刺激的细胞反应、细胞组分、胞外区和酶调节活性等。这些差异蛋白表达模式的变化表明，卵黄蛋白原（Vtg 3）、蛋白S100（S100 A10）、17-β羟类固醇脱氢酶（HSD 17 B1）、pentaxin、透明质酸（ZP3.2）、延伸因子1-α、caluemnin B和14-3-3蛋白可能在斑马鱼最终卵母细胞成熟过程中发挥特异性作用。这些数据将为理解斑马鱼卵母细胞成熟的分子机制提供有力的信息，这些蛋白可能作为标记物来预测斑马鱼卵母细胞的成熟。(C)2017爱思唯尔公司All rights reserved.
The molecular mechanisms underlying final oocyte maturation in zebrafish (Danio rerio) remain poorly understood. The present study aimed to employ iTRAQ approach for a comprehensive characterization of during zebrafish oocyte maturation proteome and for comparison between fully-grow immature and mature oocytes prior to ovulation. A total of 1568 proteins were identified, which was representing the largest zebrafish isolated oocytes proteome dataset to date. Differential expression analysis revealed 190 proteins significantly changes between immature and mature oocytes, which 136 proteins were up regulated and 54 proteins were down-regulated in mature oocytes comparison with immature oocytes. Functional analysis revealed that these differential proteins were mostly involved in cellular response to estrogen stimulus, cellular components, extracellular region, and enzyme regulator activity, etc. The revealed differentially changes in protein expression patterns associated with oocyte maturation suggest that several of the examined proteins, such as vitellogenin(Vtg3), protein S100(S100A10), 17-beta hydroxysteroid dehydrogenase(HSD17B1), pentaxin, zona pellucida (ZP3.2), elongation factorl-alpha, caluemnin B, and 14-3-3 protein may play a specific role during zebrafish final oocyte maturation. These data will provide powerful information for understanding the molecular mechanism underlying zebra fish oocyte maturation, and these proteins may potentially act as markers to predict control oocyte maturation of zebrafish oocytes. (C) 2017 Elsevier Inc. All rights reserved.