Biochemical and structural factors contributing to seasonal variation in the texture of farmed atlantic halibut (Hippoglossus hippoglossus L.) flesh
Biochemical and structural factors contributing to seasonal variation in the texture of farmed atlantic halibut (Hippoglossus hippoglossus L.) flesh
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DOI：
10.1021/jf063614h
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发表时间：
2007-07-11
影响因子：
6.1
通讯作者：
Johnston, Ian A.
中科院分区：
文献类型：
--
作者：
Hagen, Orjan;Solberg, Christel;Johnston, Ian A.
影响鱼肉质地的因素包括pH值、含水量、快肌纤维密度、胶原蛋白和羟赖氨酰吡啶啉（PYD）交联的浓度，在商业化养殖的大西洋比目鱼（Hippoglossus pigglossus L.）中进行了僵硬后的研究。从2004年5月到2005年5月，在12个月的时间里，每季度对鱼类进行采样。羟脯氨酸（HYP）作为胶原蛋白和PYD的量度，使用高效液相色谱（HPLC）法测定。以叉长和季节为协变量的ANCOVA模型用于探索质地、pH、肌纤维密度、碱不溶性胶原蛋白（a-i HYP）、碱溶性胶原蛋白（a-s HYP）和PYD交联的季节效应。多元线性回归（MLR）分析表明，对质构影响最大的因素是PYD >水分（%）> a-iHYP>纤维密度，而pH和a-sHYP与质构无相关性。快肌纤维密度纹理的贡献被发现性别之间和季节，男性和春季贡献更多。影响质地的最重要参数是PYD，在线性回归分析中解释了总变异的64%（p < 0.001）。它的结论是，交联过程是非常重要的刚性和强度的胶原蛋白在大西洋大比目鱼肉。养殖大比目鱼应在秋季或初冬收获，此时质地和营养良好，以获得最佳质量。
Factors contributing to the texture of fish flesh, including pH, water content, density of fast muscle fibers, and the concentration of collagen and hydroxylysyl pyridinoline (PYD) cross-links, were investigated post-rigor in commercially farmed Atlantic halibut (Hippoglossus hippoglossus L.). The fish was sampled every quarter for a 12 month period from May 2004 to May 2005. Hydroxyproline (HYP) as a measure of collagen and PYD were determined using a high-performance liquid chromatography (HPLC) method. An ANCOVA model with fork length and season as covariates were used to explore the seasonal effects on texture, pH, muscle fiber density, alkaline-insoluble collagen (a-i HYP), alkaline-soluble collagen (a-s HYP), and PYD cross-links. A multiple linear regression (MLR) showed that the most important factors contributing to texture were PYD > water (%) > a-i HYP > fiber density, while pH and a-s HYP did not show any correlation to texture. The contribution of fast muscle fiber density to texture was found to vary between sexes and with the season, contributing more in males and in the spring. The most important parameter affecting texture was PYD, explaining 64% (p < 0.001) of the total variation in a linear regression analysis. It is concluded that cross-linking processes are of great importance for the rigidity and strength of the collagen in Atlantic halibut flesh. Farmed halibut should be harvested in the fall or early winter when texture and nutrition are good to obtain optimal quality.