The oligodeoxynucleotide probes for the site-specific modification of RNA
The oligodeoxynucleotide probes for the site-specific modification of RNA
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用于RNA位点特异性修饰的寡脱氧核苷酸探针
DOI：
10.1039/c1cs15066a
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发表时间：
2011
影响因子：
46.2
通讯作者：
Yosuke Taniguchi
中科院分区：
文献类型：
--
作者：
Shigeki Sasaki;Kazumitsu Onizuka;Yosuke Taniguchi
随着对RNA生物学功能的了解不断扩大，对研究细胞内RNA的研究工具的需求也在增加。寡核苷酸可以针对靶RNA序列进行合理的设计，因此，已成为开发RNA特异性分子的可靠平台。RNA的化学修饰对RNA的研究有很大的影响；RNA的荧光标记有助于监测RNA的生产、加工、在细胞内的重新定位、与其他细胞内成分的相互作用和降解等。化学修饰可能通过多种途径影响RNA的功能，因此，在生物研究、治疗方法和人工操纵RNA功能方面具有潜在的应用价值。本教程综述从介绍修饰RNA的生物学相关性开始，重点介绍了用于RNA共价修饰的寡脱氧核苷酸探针的最新进展。并对这一新技术的发展前景进行了展望。
As the knowledge of the biological functions of RNA expands, the demand for research tools to investigate intracellular RNA is increasing. Oligonucleotides can be rationally designed for the target RNA sequence, and therefore, have become a reliable platform for the development of specific molecules for RNA. The chemical modification of RNA has a strong impact on RNA research; the fluorescent labeling of RNA is useful to monitor RNA production, processing, relocation in the cell, interaction with other intracellular components and degradation, etc. Chemical modification may affect the RNA function through a variety of pathways, and therefore, would be potentially useful for biological research, therapeutic approach and artificial manipulation of the RNA function. This tutorial review starts with an introduction of the biological relevance of modified RNA, and focuses on the recent progress of the oligodeoxynucleotide probes for the covalent modifications of RNA. The prospects of this new technology are also discussed.