Novel receptor-like kinase ALE2 controls shoot development by specifying epidermis in Arabidopsis
Novel receptor-like kinase ALE2 controls shoot development by specifying epidermis in Arabidopsis
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DOI：
10.1242/dev.003533
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发表时间：
2007-05-01
期刊：
影响因子：
4.6
通讯作者：
Machida, Yasunori
中科院分区：
文献类型：
--
作者：
Tanaka, Hirokazu;Watanabe, Masaru;Machida, Yasunori
表皮在动物和植物的各种器官的发育和保水中发挥着至关重要的作用。在拟南芥中，枯草杆菌酶ABNORMAL LEAF SHAPE 1（ALE1）和拟南芥Crinkly 4（ACR 4）受体样蛋白激酶（RLK）的同源物参与了表皮表面功能所需的细胞间通讯。我们在拟南芥中发现了一个新的突变基因ale 2，它与各种表皮缺陷相关，包括表皮相关组织的解体、叶角质层缺陷和器官融合。ALE2编码一个先前未表征的RLK，其具有一簇碱性氨基酸残基，随后在推定的细胞外结构域中具有含半胱氨酸的序列。我们的遗传学研究表明，ALE2和ACR 4在同一过程中的功能，而ALE1具有不同的作用模式，这三个基因发挥部分重叠的作用，积极调节protodermspecific基因的表达和叶器官的形成。我们建议，至少有两种模式的细胞间通讯促进表皮的规格，从而促进芽器官在拟南芥。
The epidermis plays crucial roles in the development of various organs and in water retention in both animals and plants. In Arabidopsis thaliana, the subtilase ABNORMAL LEAF SHAPE 1 (ALE1) and the Arabidopsis homolog of the Crinkly4 (ACR4) receptor-like protein kinase (RLK) have been implicated in the intercellular communication that is required for surface functions of the epidermis. We have identified a novel mutant gene in Arabidopsis, ale2, which is associated with various epidermal defects, including disorganization of epidermis-related tissues, defects in the leaf cuticle and the fusion of organs. ALE2 encodes a previously uncharacterized RLK with a cluster of basic amino acid residues followed by a cysteine- containing sequence in the putative extracellular domain. Our genetic investigations suggest that ALE2 and ACR4 function in the same process, whereas ALE1 has a different mode of action, and that these three genes play partially overlapping roles in positively regulating protodermspecific gene expression and for the formation of leafy organs. We propose that at least two modes of intercellular communication facilitate the specification of epidermis, thereby promoting shoot organogenesis in Arabidopsis.