Protein Dynamics in Young Maize Root Hairs in Response to Macro- and Micronutrient Deprivation.
Protein Dynamics in Young Maize Root Hairs in Response to Macro- and Micronutrient Deprivation.
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DOI：
10.1021/acs.jproteome.5b00399
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发表时间：
2015-07
影响因子：
4.4
通讯作者：
Zhi Li;D. Phillip;B. Neuhäuser;W. Schulze;U. Ludewig
中科院分区：
文献类型：
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作者：
Zhi Li;D. Phillip;B. Neuhäuser;W. Schulze;U. Ludewig
植物用根毛增加根表面积，以提高从土壤中获取养分的能力。根毛的单细胞特性和它们在根表面的位置使它们成为研究与土壤溶液直接接触的根皮细胞的适应过程的有吸引力的系统。在玉米幼苗中，根被根毛密集地覆盖，尽管种子中的营养储备足以支持幼苗生长几天。我们使用了一种无标记的定量蛋白质组学方法来研究在存在和不存在几种宏量和微量营养素的情况下，在气培培养中生长的4天龄根毛中蛋白质丰度的调整。与全营养条件下发育的根毛蛋白质组相比，在与大量营养素（N、P、K和Mg）缺乏相关的途径中观察到蛋白质丰度的变化。例如，培养基中缺乏N抑制初级N代谢途径，增加氨基酸合成，但抑制其降解，并影响初级碳代谢，如糖酵解。糖酵解同样受到钾和磷剥夺的影响，但糖酵解途径负调控的微量营养素铁和锌的情况下。与此相反，锰的剥夺对根毛蛋白质组几乎没有影响。我们的研究结果表明，无论是非常年轻的根毛的代谢调整细胞营养不足，已经经历过或根毛感觉外部缺乏特定的营养素的营养液，并相应地调整其代谢。
Plants increase their root surface with root hairs to improve the acquisition of nutrients from the soil. The unicellular character of root hairs and their position at the root surface make them an attractive system to investigate adaptive processes of rhizodermal cells that are in direct contact with the soil solution. In young maize seedlings, roots are densely covered with root hairs, although nutrient reserves in the seed are sufficient to support seedling growth rates for a few days. We used a label-free quantitative proteomics approach to study protein abundance adjustments in 4 day old root hairs grown in aeroponic culture in the presence and absence of several macro- and micronutrients. Compared to the proteome of root hairs developed under full nutrition, protein abundance changes were observed in pathways related to macronutrient (N, P, K, and Mg) deficiencies. For example, lack of N in the medium repressed the primary N metabolism pathway, increased amino acid synthesis, but repressed their degradation, and affected the primary carbon metabolism, such as glycolysis. Glycolysis was similarly affected by K and P deprivation, but the glycolytic pathway was negatively regulated by the absence of the micronutrients Fe and Zn. In contrast, the deprivation of Mn had almost no affect on the root hair proteome. Our results indicate either that the metabolism of very young root hairs adjusts to cellular nutrient deficiencies that have been already experienced or that root hairs sense the external lack of specific nutrients in the nutrient solution and adjust their metabolism accordingly.